摘要
为评估陕西省榆林市O型、AsiaI型和A型口蹄疫的免疫抗体水平,比较单次免疫和加强免疫后的抗体水平和变化趋势,在陕西省榆林市采集55个规模场和120个散养户强制免疫后的猪、牛、羊血清共计2310份,用液相阻断ELISA法检测口蹄疫免疫抗体。结果显示:羊O型、Asia?型,猪O型,牛O型、AsiaI型和A型口蹄疫平均免疫抗体合格率分别为(57.12±4.8)%、(64.66±4.2)%、(66.46±2.02)%、(56±5.98)%、(57.9±1.66)%和(64.29±4.26)%;规模场中,只有羊AsiaI型和猪O型口蹄疫抗体合格率达到70%以上,而散养户的全部口蹄疫抗体合格率均低于70%,差异极显著(P≤0.01);规模场首免1个月后进行加强免疫,发现抗体合格率全部由低于70%升高至80%以上,差异显著(P≤0.01)。结果表明,榆林市的整体口蹄疫免疫效果较差,只有规模场的羊AsiaI型和猪O型口蹄疫抗体合格率达到70%以上,因此需要对这些免疫不合格畜群进行加强免疫,只有这样方能实现有效的免疫保护。本研究为了解本地区口蹄疫免疫效果,以及免疫程序制定和进一步做好口蹄疫防控工作提供了参考。
In order to evaluate the immune antibody level of foot-and-mouth disease(FMD))of type O,Asia I and A,and to compare the level and trend of antibodies after first and reinforced vaccination,a total of 2 310 serums samples were collected from pigs,cattle and sheep with compulsory vaccination in 55 large-scale farms and 120 freerange households in Yulin City of Shaanxi Province,and the immune antibodies were detected by liquid-phase blocking ELISA.The results showed that the average qualified rates of antibodies against types O and Asia?in sheep,type O in pigs,and types O,Asia?and A in cattle were(57.12±4.8)%,(64.66±4.2)%,(66.46±2.02)%,(56±5.98)%,(57.9±1.66)%,(64.29±4.26)%,respectively;in scaled farms,only the qualified rate of type Asia?in sheep and type O in pigs reached up to more than 70%,while the rates were all less than 70%in free-range households,and the difference was extremely obvious(P≤0.01).For the scaled farms,the qualified rates of all kinds of antibodies 7 2019年第36卷第8期increased from less than 70%to more than 80%when the reinforced vaccination was conducted one month after first vaccination,with obvious difference(P≤0.01).In conclusion,the overall effects of FMD vaccination in Yulin City were relatively poor,hence it was necessary to strengthen vaccination in these herds to enhance the vaccination effects.The research would provide some reference for recognizing the effect of FMD vaccination,formulating immune procedures and furthering the prevention and control of FMD.
作者
王雅媛
白崇生
权富生
刘健鹏
Wang Yayuan;Bai Chongsheng;Quan Fusheng;Liu Jianpeng(Northwest A&F University,Yangling,Shaanxi 712100,China;Yulin Animal Disease Control Center,Yulin,Shaanxi719000,China)
出处
《中国动物检疫》
CAS
2019年第8期6-10,共5页
China Animal Health Inspection
基金
陕西省农业科技创新重点项目(NYKJ-2015-081)
陕西省农业科技创新转化项目(NYKJ-2015-023)
关键词
口蹄疫
免疫抗体
猪
牛
羊
榆林市
foot-and-mouth disease
immune antibody
pig
cattle
sheep
Yulin City
