摘要
目的比较颈动脉内膜空气干燥法及套环法建立兔粥样硬化斑块模型的优劣。方法 20只新西兰白兔随机分为空气干燥法组(A组)和套环法组(B组),分别行颈动脉内膜空气干燥术及颈动脉外膜硅胶套环术,术后高脂喂养。术后0周、4周、8周采用ELISA检测血浆中总胆固醇(TC)、甘油三酯(TG)及低密度脂蛋白(LDL)浓度;术后4周、8周、12周采用常规二维超声检查兔颈总动脉情况。术后12周完成超声检查后,对2组兔颈总动脉斑块行HE染色,病理学观察。结果高脂饮食4周后血清TG、TC、LDL的浓度升高(P<0.05),并持续升高,而同一时间点A组与B组间上述指标未见明显差别;高脂喂养8周后超声检查见2组兔的损伤段颈总动脉内中膜增厚,管壁现斑块强回声;病理学观察见大量泡沫细胞,且套环组上述病变较空气组更早更显著。结论颈总动脉套环法使兔颈总动脉在更短时间内出现部位明确预定的斑块,安全性和有效性优于内膜空气干燥法。
Objective To compare the effect of air-drying and collar method on establishment of carotid atherosclerotic plaque in rabbits. Methods Rabbits were randomly divided into two groups: an air-drying group and a collar group with 10 subjects each. After operations, they were fed with fat-rich diet. The plasma levels of total cholesterol(TC), triglyceride(TG) and low density lipoprotein(LDL)were measured by ELISA in the 0 th, 4 th, 8 th week, and the carotid artery was scanned by ultrasound in the 4 th, 8 th, 12 th week. All rabbits were sacrificed at the end of experiment time. The morphology of the plaques was observed after HE staining. Results With high fat diet, the injured carotid of both groups were thickened and attached by strongly echoed plaques. HE staining showed a large number of foam cells, and all of these phenomenon showed earlier and more serious in the silicone collar group when compared with the air-drying group. Conclusion Carotid artery silicone collar method is safer and more effective than air-drying way in leading to the formation of atheromatous plaque.
作者
王琮
陈筱雪
杨少玲
王凤翎
范林艳
贺倩倩
WANG Cong;CHEN Xiao-xue;YANG Shao-ling;WANG Feng-ling;FAN Lin-yan;HE Qian-qian(Shanghai Fengxian Central Hospital Affiliated to AnHui University of Science and Technology,Shanghai 201400,China)
出处
《实验动物与比较医学》
CAS
2019年第3期208-212,共5页
Laboratory Animal and Comparative Medicine
基金
上海市卫生与计划生育委员会课题面上项目(201740053)
上海市科学技术委员会引导项目(18411970000)
