摘要
目的比较16S rRNA序列分析法与传统生化分析法鉴别54株分枝杆菌模式菌株的异同。方法采用PCR方法扩增54株分枝杆菌模式菌株16S rRNA序列,通过序列比对构建发育树状谱,计算相似性,并与传统生化分析分型结果进行比较。结果16S rRNA序列分析可鉴定出41株(41种)分枝杆菌,其它6组(包括13株11种)的分枝杆菌16S rRNA序列两两之间相同无法区别,传统的生化分析方法难以区分鸟分枝杆菌和胞内分枝杆菌、龟分枝杆菌和偶然分枝杆菌,但采用16S rRNA很容易区分;16S rRNA基因无法区分海分枝杆菌与溃疡分枝杆菌、堪萨斯分枝杆菌和胃分枝杆菌,但采用传统生化分型方法很容易区分。结论在分枝杆菌的分型鉴定方面,分枝杆菌16S rRNA基因序列分析与传统分型均十分有效,且两者的联合应用,能够提高鉴定的准确性。
Objective To analyze the difference between 16S rRNA sequence analysis and traditional biochemistry method for identifying mycobacteria.Methods 16S rRNA sequence analysis was used to analyze the genotype of 54 reference strains of mycobacteria,construct phylogenetic tree and calculate similarity.Its results were compared with those detected by traditional biochemistry method.Results All 41 mycobacteria strains(total 41 kinds)could be discriminated for the difference of 16S rRNA except 6 couple of mycobacteria(including 11 kinds and 13 strains)which have the same 16S rRNA sequences between each other.M.avium and M.intracellulare,M.chclonae.chelonae and M.fortuitum were difficult to be identified by traditional biochemistry method but easy to be discriminated by 16S rRNA gene sequence grouping.M.ulcerans and M.marinum,M.gastri and M.kansasii could not be identified with 16S rRNA gene sequence analysis but could be discriminated by traditional biochemistry method.Conclusions 16S rRNA gene sequence analysis and biochemistry method were accurate and efficient to discriminate Mycobacteria.The combination of the two methods can improve the accuracy of identification.
作者
叶雅丽
闫李侠
黄至澄
陈保文
王国治
YE Yali;YAN Lixia;HUANG Zhicheng(Department of Laboratory Medicine,Huangyan Hospital of Wenzhou Medical University,Taizhou First People’s Hospital,Taizhou 318020,China)
出处
《全科医学临床与教育》
2019年第7期591-594,I0001,共5页
Clinical Education of General Practice
基金
“十二五”国家重大科技专项(2012ZX10004702)
