摘要
目的分析变应性鼻炎(AR)患者外周血单个核细胞(PBMCs)中T细胞免疫球蛋白域黏蛋白域蛋白-3(TIM-3)的表达及其与调节性T细胞(Treg)的关系,探讨其在AR发病中的作用。方法选取2017年5-11月就诊于锦州医科大学附属第一医院的AR患者(AR组,n=30)及健康志愿者(HC组,n=25)。抽取外周静脉血,采用密度梯度离心法分离PBMCs,流式细胞仪检测AR组与HC组Treg及TIM-3^+Treg的百分比,分析TIM-3的表达与Treg的关系。阻断TIM-3,观察其对Treg的影响。结果 AR组外周血中Treg(CD4^+CD25^+FoxP3^+)细胞百分比(1.16%±0.13%)低于HC组(5.12%±0.11%)(Z=–6.339,P<0.01);TIM-3^+Treg细胞百分比(11.76%±1.07%)高于HC组(3.15%±0.22%)(Z=–5.570,P<0.01)。AR患者中CD4^+CD25+FoxP3^+Treg细胞的表达与其表面Tim-3的表达呈负相关(r=–0.763,P<0.05)。使用阻断剂后,AR患者外周血中Treg细胞百分比(1.67%±0.76%)与阻断前(1.44%±0.78%)比较未见明显改变(Z=–1.45,P=0.135),阻断后AR患者外周血中IL-10+Treg的百分比(2.33%±1.45%)与阻断前(1.57%±1.12%)比较明显上调(Z=–2.97,P=0.003)。结论 TIM-3可能在AR发病过程中发挥作用,该作用与Treg的失衡有关。
Objective To detect the expression of TIM-3(T cell immunoglobulin domain,mucin domain) and its relationship with Treg(regulatory T) cells isolated from peripheral blood mononuclear cells(PBMCs) in the patients with allergic rhinitis(AR),and investigate the role of TIM-3 in the occurrence and development of the allergic rhinitis inflammation.Methods AR patients(AR group,n=30) and healthy subjects(HC group,n=25) were selected as experimental group and control group respectively,and 2 ml peripheral venous bloods were collected from these two groups.Peripheral blood mononuclear cells(PBMCs)were isolated from the blood by density gradient centrifugation.The proportion of CD4^+CD25+FoxP3+,reflecting expression of Treg,and TIM-3^+Treg was detected by flow cytometry.The relationship between TIM-3 expression and Treg cells was analyzed.The expression of TIM-3 on the surface of CD4^+CD25^+FoxP3^+ Treg cells was analyzed by flow cytometry.Then the TIM-3 expression was blocked,and its influence on Treg cells was observed.Results The percentage of Treg cells in peripheral blood of patients with AR(1.16%±0.13%) was lower than that of healthy controls(5.12%±0.11%)(Z=–6.339,P<0.01).The expression of TIM-3 on the surface of Treg cells in AR patients(11.76%±1.07%) was higher than that in healthy controls(3.15%±0.22%)(Z=–5.570,P<0.01).The expression of CD4^+CD25^+FoxP3^+Treg cells in AR patients was negatively correlated with the expression of TIM-3 on the surface(r=–0.763,P<0.05);The percentage of Treg cells in peripheral blood of AR patients after the block(1.67%±0.76%)was significantly higher than that before block(1.44%±0.78%)(Z=–1.45,P=0.135).The percentage of IL-10 secreted by Treg cells in peripheral blood after block(2.33%±1.45%) was higher than that before block(1.57%±1.12%)(Z=–2.97,P=0.003) in AR patients.Conclusion The expression of TIM-3 in PBMCs may increase in patients with AR,TIM-3 may play an important role in the pathogenesis of AR,and may be related to the imbalance of Treg cells.
作者
徐子琴
王帅
汪瞾
何韶衡
张慧云
陈冬
XU Zi-qin;WANG Shuai;WANG Zhao;HE Shao-heng;ZHANG Hui-yun;CHEN Dong(Department of Otorhinolaryngology Head and Neck Surgery, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, China;Allergy and Clinical Immunology Research Centre, the First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning 121001, China)
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2019年第7期611-614,共4页
Medical Journal of Chinese People's Liberation Army
基金
辽宁省自然科学基金(20180550039)~~
