摘要
目的探讨致死型约氏疟原虫(Plasmodium yoelii 17XL,P.y 17XL)红内期感染对肝脏的作用影响。方法采用新鲜P.y 17XL感染红细胞(1×10^6)腹腔感染BALB/c小鼠,定期尾静脉取血检测红细胞感染率,观察小鼠生存率。取感染率30%、50%和70%小鼠肝组织,进行HE染色、F4/80免疫组织化学分析(IHC)和疟色素含量检测。密度梯度离心法分离肝单个核细胞。流式细胞术检测肝脏巨噬细胞数量。结果 P.y 17XL感染小鼠红细胞感染率从第3 d(7.1%)开始快速上升,第7 d达峰值(81.5%)后小鼠全部死亡(生存率0)。HE染色显示疟色素在P.y 17XL感染小鼠肝脏大量沉积,肝组织结构紊乱。免疫组化显示,F4/80+巨噬细胞胞浆富含大量疟色素颗粒。与对照组相比,肝脏疟色素含量在30%组(6.079μmol/L)、50%组(5.35μmol/L)和70%组(8.542μmol/L)明显增加(均P<0.001),且70%组明显高于30%组和50%组(均P<0.05)。流式分析显示,与对照组相比,肝脏F4/80^+CD11b^+CD11c^-巨噬细胞百分含量在30%组(7.81%,P<0.01)、50%组(6.41%,P<0.05)和70%组(4.35%,P<0.05)均显著升高(图5A/B),绝对计数亦明显增加(均P<0.05)。结论 P.y 17XL红内期感染的疟色素通过激活巨噬细胞介导肝脏病理损伤。
Objective To investigate the effects of infection with a lethal Plasmodium yoelii strain(17 XL) on the liver. Methods BALB/c mice were intraperitoneally administered fresh RBCs infected with P. yoelii 17 XL(1×10^6). Blood samples were taken from the tail vein to detect parasitemia, and the survival rate were observed. The liver tissues of mice infected with P. yoelii 17 XL(30%, 50%, or 70% parasitemia) were examined using HE staining, F4/80 immunohistochemistry, and quantification of the hemozoin concentration. Hepatic mononuclear cells were isolated using gradient density centrifugation. The percentage and absolute number of macrophages were analyzed using flow cytometry. Results Parasitemia increased markedly after day 3(7.1%), and all mice died after the peak(81.5%) on day 7. The results of HE staining indicated that P. yoelii 17 XL deposited hemozoin in the livers of infected mice. The results of IHC indicated that F4/80^+ macrophages had an abundance of hemozoin particles. The concentration of hemozoin increased significantly in the 30% parasitemia group(6.079 μmol/L)(P<0.001), 50% parasitemia group(5.35 μmol/L)(P<0.001), and 70% parasitemia group(8.542 μmol/L) compared to that in the control group(P<0.001);the concentration in the 70% parasitemia group was markedly higher than that in both the 30% parasitemia group and the 50% parasitemia group. Flow cytometry indicated that the percentage of F4/80^+CD11 b^+CD11^c-macrophages was markedly higher in the 30% parasitemia group(7.81%, P<0.01), 50% parasitemia group(6.41%, P<0.05) and 70% parasitemia group(4.35%, P<0.05) compared to that in the control group. The absolute number of macrophages increased markedly in mice infected with P. yoelii 17 XL(P<0.05). Conclusion Hemozoin produced as a result of a P. yoelii 17 XL infection induced pathological liver damage by activating macrophages.
作者
黄旭
吕童
曹雅明
冯辉
HUANG Xu;LV Tong;CAO Ya-ming;FENG Hui(Department of Immunology, College of Basic Medical Sciences, China Medical University,Shenyang,China 110122)
出处
《中国病原生物学杂志》
CSCD
北大核心
2019年第6期671-674,699,共5页
Journal of Pathogen Biology
基金
辽宁省高等学校杰出青年学者成长计划项目(No.LJQ2014081)
关键词
疟疾
疟原虫
约氏
巨噬细胞
疟色素
Malaria
parasite
Plasmodium yoelii
macrophages
hemozoin
