摘要
目的了解大连地区流行的GⅡ型诺如病毒基因特征及其分布,为预防控制诺如病毒流行提供依据。方法采用荧光定量RT-PCR方法对2015年大连地区哨点医院上送的1 253份食源性腹泻病例粪便标本进行GⅡ型诺如病毒检测,对部分阳性标本进行亚型检测和基因特征分析。结果 1 253份标本中,检出GⅡ诺如病毒20份,阳性率为1.6%,其中8株GⅡ型诺如病毒经ORF1和ORF2连接区扩增和测序,发现5株为GⅡ.17,3株为GⅡ.4。进化树分析发现,本次大连地区检出的GⅡ.17和GⅡ.4分别与2015年韩国株和2015年中国台湾株亲缘关系密切。结论大连地区存在GⅡ.17和GⅡ.4诺如病毒流行,GⅡ.17诺如病毒在大连地区首次被检出。GⅡ.17是否是大连地区的优势流行株,还有待进一步跟踪监测。
Objective To study the genetic characteristic and distribution of norovirus GⅡin Dalian,so as to provide references for the prevention and control of norovirus.Methods A total of 1 253 stool specimens from patients with food borne diarrhea were collected from sentinel hospitals in 2015 and detected by real-time RT-PCR.The genotypes and genetic characteristic of some norovirus GⅡ were determined and analyzed.Results Among 1 253 stool specimens,20 cases were positive for norovirus GⅡ,the positive rate was 1.6%.The genotypes of 8 strains norovirus GⅡ were determined,based on sequences of ORF1-ORF2 joining region,5 strains were GⅡ.17,3 strains were GⅡ.4.The phylogenetic tree analysis revealed that the GⅡ.17 and GⅡ.4 found in Dalian showed a close relationship with those from south Korea and Taiwan in 2015,respectively.Conclu￣sion There are norovirus GⅡ.17 and GⅡ.4 epidemics in Dalian,and this is the first time to detect GⅡ.17 in Dalian region.Whether GⅡ.17 was epidemic predominant in Dalian needs further tracing monitoring.
作者
栾明春
郎兴莹
于蕾
薄志坚
LUAN Ming-chun;LANG Xing-ying;YU Lei;BO Zhi-jian(Dalian Center for Disease Control and Prevention,Dalian,Liaoning 116021,China)
出处
《中国卫生检验杂志》
CAS
2019年第13期1561-1563,共3页
Chinese Journal of Health Laboratory Technology
基金
大连市医学科学研究计划项目(1811099)
