摘要
目的: TH287作为MutT同系物1(MutT homolog1,MTH1)蛋白的抑制剂,可有效抑制癌细胞中过表达的MTH1蛋白,从而解除对下游凋亡相关蛋白半胱氨酰天冬氨酸特异性蛋白酶3 (cysteinyl aspartate-specific protease-3,Caspase-3)介导细胞凋亡的抑制作用。本研究旨在检测TH287对口腔鳞状细胞癌CAL27细胞增殖性和迁移性的抑制作用。方法:通过噻唑蓝(methyl thiazol tetrazolium,MTT)法检测TH287对口腔鳞状细胞癌CAL27细胞增殖性的抑制作用并确定药物最适浓度;通过流式细胞术检测TH287对CAL27细胞凋亡的促进作用;通过Western blot实验检测TH287对MTH1蛋白表达的抑制作用;通过划痕实验和Transwell迁移实验检测TH287对CAL27细胞迁移性的抑制作用。结果: TH287有效地抑制了CAL27细胞的增殖性,其最适浓度为 100μmol/L 。TH287同时也促进CAL27细胞发生凋亡并抑制MTH1蛋白的表达。除此之外,CAL27细胞的迁移性也受到了抑制。结论: TH287可以抑制CAL27细胞的增殖并促进其凋亡。与此同时,CAL27细胞的迁移性也明显受到抑制。TH287对CAL27细胞增殖性和迁移性的抑制作用可能与MTH1蛋白表达受到抑制有关。因此,TH287可能成为口腔鳞状细胞癌靶点治疗的新药物。
Objective: To test the inhibition of TH287 on the proliferation and metastasis of CAL27.Methods: MTT assay was used to test the inhibition of TH287 on the proliferation of CAL27 and ascertain the appropriate concentration.The promotion of TH287 on the apoptosis of CAL27 was tested by the flow cytometry.The inhibition of TH287 on the MTH1 expression was examined by the western blot.The inhibition of TH287 on the metastasis of CAL27 was examined by the scratch test and Transwell (migration).Results: TH287 could inhibit the proliferation of CAL27 effectively and the appropriate concentration was 100 μM.TH287 could also promote the apoptosis of CAL27 and inhibit the MTH1 expression.In addition,the metastasis of CAL27 was also inhibited by the TH287.Conclusion: TH287 could inhibit the proliferation and promote the apoptosis of CAL27.Meanwhile,the metastasis of CAL27 was also inhibited by TH287.The inhibition of TH287 on the proliferation and metastasis of CAL27 may be caused by the suppression of MTH1 expression.Therefore,TH287 has great potential to be a new drug in targeted therapy.
作者
单秋生
李国林
许嘉琪
SHAN Qiusheng;LI Guolin;XU Jiaqi(Department of Oral and Maxillofacial Surgery,Harbin Medical University,Harbin 150001,China)
出处
《口腔医学研究》
CAS
北大核心
2019年第7期704-707,共4页
Journal of Oral Science Research
基金
黑龙江省自然科学基金(编号:H2018041)
