摘要
目的探讨荔枝核提取液预处理对小鼠急性酒精性肝损伤的缓解作用。方法将50只小鼠随机分为正常对照组、模型组、联苯双酯(0.2g/kg)组、荔枝核低剂量(20g/kg)组和荔枝核高剂量(40g/kg)组,每组10只。正常对照组和模型组给予0.2mL/10g生理盐水灌胃,其余各组予相应剂量药物灌胃,连续给药20d。末次给药2h后,除正常对照组外,其余各组均采用56°白酒灌胃构建急性酒精性肝损伤模型。建模12h后,检测各组小鼠血清ALT和AST水平,血清和肝组织超氧化物歧化酶(SOD)和丙二醛水平,并镜下观察肝脏组织形态学变化。结果与正常对照组比较,模型组ALT、AST、丙二醛水平升高,SOD水平降低(均P<0.05),显微镜下可观察到小叶中央静脉淤血、扩张,肝细胞弥漫性水肿、胞浆疏松化及气球样变,部分肝细胞溶解坏死。与模型组比较,荔枝核低、高剂量组血清ALT、AST水平均降低,且荔枝核高剂量组血清ALT、AST水平低于荔枝核低剂量组(均P<0.05);荔枝核高剂量组血清和肝组织SOD水平升高而丙二醛水平降低(P<0.01);荔枝核低、高剂量组肝组织形态学有较大改善。结论荔枝核提取液预处理对小鼠急性酒精性肝损伤具有一定缓解作用,其机制可能与荔枝核提取液提高机体对自由基的防御能力和减轻氧化应激有关。
Objective To explore the efficacy of preconditioning with lychee seed extract for ameliorating acute alcoholic liver injury in mice. Methods Fifty mice were randomly divided into normal control group,model group,biphenyl diester(0.2 g/kg) group,low-dose(20 g/kg) lychee seed group and high-dose(40 g/kg) lychee seed group,with 10 mice in each group.The normal control group and the model group were given 0.2 mL/10 g normal saline by gavage for consecutive 20 days,and the remaining groups were given corresponding doses of drug by gavage for consecutive 20 days.Two hours after the last administration,except the normal control group,the remaining groups received 56 percent liquor by gavage to develop the model of acute alcoholic liver injury.Twelve hours after modeling,serum ALT and AST levels,as well as serum and liver tissue superoxide dismutase(SOD) and malondialdehyde levels ,were measured in each group,and the morphological changes in liver tissue were observed under microscope. Results Compared with the normal control group,the model group had elevated ALT,AST and malondialdehyde levels and decreased SOD level(all P <0.05),microscopically,presenting congestion and swelling in central vein of the lobule,diffuse edema,loose cytoplasm and ballooning degeneration in hepatocytes,and lytic necrosis in some hepatocytes. Compared with the model group,the low- and high-dose lychee seed groups had lower serum ALT and AST levels,and the serum levels of ALT and AST in the high-dose lychee seed group were lower than those in the low-dose lychee seed group(all P <0.05);the high-dose lychee seed group exhibited increased SOD levels and decreased malondialdehyde levels in serum and liver tissue( P <0.01);fairly great morphological improvements in liver tissue were observed in the low- and high-dose lychee seed groups. Conclusion Preconditioning with lychee seed extract has a certain efficacy for ameliorating acute alcoholic liver injury in mice,its mechanism might be related to lychee seed extract′s improving the ability to de
作者
黄景珠
莫庸
黄继杰
黎璇
何幸娟
李晓潇
方晓燕
黄丽娟
HUANG Jing-zhu;MO Yong;HUANG Ji-jie;LI Xuan;HE Xing-juan;LI Xiao-xiao;FANG Xiao-yan;HUANG Li-juan(School of Clinical Medicine,Baise 533000,China;Experimental and Teaching Center of Morphology,Baise 533000,China;Department of Physiology,Youjiang Medical College for Nationalities,Baise 533000,China)
出处
《广西医学》
CAS
2019年第11期1406-1409,共4页
Guangxi Medical Journal
基金
右江民族医学院创新创业训练计划项目(201710599103)
关键词
酒精性肝损伤
急性
荔枝核
提取液
预处理
小鼠
Alcoholic liver injury
Acute
Lychee seed
Extract
Preconditioning
Mouse
