摘要
有效抑制或淘洗亚硝酸盐氧化菌(NOB)是短程硝化-厌氧氨氧化(PN/A)工艺应用于城市污水处理的关键.以因NOB大量增长受到破坏的城市污水PN/A系统为对象(硝酸盐(NO3^--N)生成比例为0.90),考察了羟胺(NH 2OH)投加浓度和投加方式对其恢复的效果.结果显示,当序批式反应器中初始NH2OH投加浓度为10mg/L时,每天投加1次,连续投加20d后,NO3^--N生成量占NH 4^+-N消耗量的比例由0.90逐步降低至0.11.表明NH 2OH(10mg/L)可原位恢复PN/A工艺.NH2OH停止投加59d后,出水NO3^--N生成比例再次小幅度上升至0.15,此时继续投加5d NH2OH(10mg/L),PN/A工艺运行良好,因此间歇投加NH 2OH可以维持PN/A工艺稳定运行.实时定量PCR结果表明,在投加NH 2OH(10mg/L)后,NOB的丰度不断下降,从(4.52±0.44)×10^10copies/g VSS(第6d)下降到(2.30±0.80)×10^9copies/g VSS(第157d),说明NH 2OH的投加有利于抑制和淘洗NOB.
The effective inhibition or wash-out of nitrite oxidizing bacteria(NOB)is the key challenge for the application of partial nitritation/anammox(PA/N)process in domestic wastewater treatment.The deteriorated domestic wastewater PN/A system was used to investigate the recovery effect of hydroxylamine(NH2OH)concentration and dosing mode on it.The nitrate(NO3^--N)production ratio of the PN/A system was 0.90,which was due to the overgrowth of NOB.The results showed that when the initial NH2OH concentration was 10mg/L in the sequencing batch reactor(SBR)and NH2OH was added once a day,the ratio of NO3^--Nproducted/NH4^+-Nconsumed was decreased from 0.90 to 0.11 after adding NH2OH for 20d.It indicated that NH2OH(10mg/L)addition could restore PN/A process in-situ.The ratio of NO3^--Nproducted/NH4^+-Nconsumed increased to 0.15 again after stopping NH2OH addition for 59d.When continued to add NH2OH into reactor for 5d,the PN/A process operated well.Therefore,the intermittent addition of NH2OH strategy could maintain the stable performance of the PN/A process.The real-time quantitative PCR results showed that the NOB abundance was decreased continuously from(4.52±0.44)×10^10 copies/g VSS(Day 6)to 9(2.30±0.80)×10^9copies/g VSS(Day 157)after NH2OH(10mg/L)addition.It indicated that NH2OH addition was beneficial to inhibit and wash out NOB.
作者
李佳
李夕耀
张琼
彭永臻
LI Jia;LI Xi-yao;ZHANG Qiong;PENG Yong-zhen(National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2019年第7期2789-2795,共7页
China Environmental Science
基金
北京市科技计划(D171100001017001)
北京市教委资助项目
