摘要
目的:探讨癌基因HuR在胃癌组织的表达和对胃癌MGC-803细胞功能的影响。方法:使用RT-qPCR分析80例临床确诊的胃癌患者胃癌组织样本中HuR的表达水平;利用pSIH载体构建敲减HuR的重组质粒,实现HuR在MGC-803细胞中的低表达,以空载体pSIH作为对照;使用划痕实验检测细胞迁移能力,使用Transwell实验检测细胞侵袭能力,使用CCK-8法检测细胞活力。结果:RT-qPCR结果显示,与癌旁对照相比,67例(84%)胃癌组织中出现HuR的表达上调;低表达HuR能显著抑制MGC-803细胞的侵袭能力、迁移能力和活力(P<0.05),提示HuR作为癌基因起作用。结论:HuR的异常表达可能与胃癌的发生发展有关,低表达HuR可以抑制胃癌MGC-803细胞的侵袭、迁移和活力。
AIM: To investigate the effects of HuR on cell function of gastric cancer cell line MGC-803. METHODS: The mRNA expression level of HuR was detected by RT-qPCR in the tumor samples of 80 gastric cancer patients diagnosed clinically. HuR gene knock-down was achieved by transfection of si-HuR into the MGC-803 cells. The invasion, migration and viability of MGC-803 cells were measured by the scratch wound hearing, Transwell and CCK-8 assays, respectively. RESULTS: High mRNA expression of HuR was observed in 67 cases(84%) of gastric cancer tissues as compared with their control samples. Furthermore, knock-down of HuR expression effectively inhibited the invasion, migration and viability of the MGC-803 cells(P<0.05), indicating that HuR play an important role in gastric cancer as an oncogene. CONCLUSION: Abnormal expression of HuR is correlated with the progression of gastric cancer. Knock-down of HuR expression inhibits the invasion, migration and viability of MGC-803 cells.
作者
曹小梅
曹楠婧
王福花
孙瑞芳
李峰
郭素堂
CAO Xiao-mei;CAO Nan-jing;WANG Fu-hua;SUN Rui-fang;LI Feng;GUO Su-tang(Department of Biochemistry and Molecular Biology, Basic Medical College, Shanxi Medical University, Taiyuan 030001, China;Department of Molecular Biology, Shanxi Provincial Cancer Institute, Taiyuan 030013, China;Sample Library, Shanxi Provincial Cancer Hospital, Taiyuan 030013, China)
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2019年第7期1328-1332,共5页
Chinese Journal of Pathophysiology
基金
山西省卫生和计划生育委员会科研基金资助项目(No.201601060)
山西省重点研发计划项目(No.201803D31166)
山西省肿瘤医院博士基金资助项目(No.2017A07)
关键词
HuR基因
胃癌
细胞活力
细胞迁移
细胞侵袭
HuR gene
Gastric cancer
Cell viability
Cell migration
Cell invasion
