摘要
目的水疱性口炎病毒纯化抗原制备兔抗VSV多克隆抗体,免疫学方法检测其结果分析比较。方法纯化的抗原制备兔抗VSV多克隆抗体,采用双向琼脂扩散试验,间接ELISA方法和中和试验测定效价。结果兔抗VSV多克隆抗体最高效价双向琼脂扩散试验为1∶32,间接ELISA法为1∶640,中和试验为1∶256。结论试验结果分析比较表明,建立纯化抗原制备的兔抗VS多克隆抗体,中和试验特异性好、敏感性高,可做为VSV的血清学检测和流行抗学调查抗供技术保障。
Objective To prepare rabbit anti-VSV polyclonal antibody by vesicular stomatitis virus purified antigen, and compare the results by immunological methods. Methods Rabbit anti-VSV polyclonal antibody was prepared by purified antigen. The titer was determined by two-way agar diffusion test, indirect ELISA method and neutralization test. Results The highest titer of the rabbit anti-VSV polyclonal antibody was 1∶32 for the two-dimensional agar diffusion test, 1∶640 for the indirect ELISA, and 1∶256 for the neutralization test. Conclusion The experimental results show that the rabbit anti-VSV polyclonal antibody prepared by the purified antigen has good specificity and high sensitivity, which can provide technical support for serological detection and epidemiological investigation of VSV.
作者
胡涛
金郁
汪学龙
李群
Hu Tao;Jin Yu;Wang Xuelong;Li Qun(Department of Microbiology, AnHui Medical University, Hefei 230032, Anhui China)
出处
《热带病与寄生虫学》
2019年第1期40-42,共3页
Journal of Tropical Diseases and Parasitology
基金
安徽省高等学校省级质量工程项目(编号2015xnzx030)
关键词
水疱性口炎病毒
中和试验
抗原纯化
多克隆抗体
Acaroid mite Vesicular stomatitis virus
Neutralization assay
Antigen purification
Polyclonal antibody
