摘要
背景:牙槽骨修复材料的种类很多,可以分为天然生物材料,人工合成材料和复合材料,各种类型的材料还可以通过化学生物等方法进行合成,其均有各自的优势和不足。目的:比较羟基磷灰石/β-磷酸三钙、纳米碳化羟基磷灰石海藻酸盐及单纯羟基磷灰石3种牙槽骨修复材料的细胞毒性及生物相容性。方法:将羟基磷灰石/β-磷酸三钙浸提液、纳米碳化羟基磷灰石海藻酸盐浸提液、羟基磷灰石浸提液分别与小鼠成骨前细胞、人成骨细胞共培养,XTT实验检测细胞线粒体活性(以单独培养的细胞为对照组),结晶紫分析实验检测细胞毒性(以DMSO培养的细胞为对照组)。取20只Wistar大鼠(购自北京维通利华实验动物有限公司),制备上颌右侧中切牙牙槽骨缺损模型,随机分4组干预:对照组不植入任何材料,其余3组分别植入羟基磷灰石/β-磷酸三钙、纳米碳化羟基磷灰石海藻酸盐及单纯羟基磷灰石材料,植入后7,21,42d,检测血清中RANKL、骨保护素质量浓度。实验已通过西南医科大学动物伦理委员会审批批准,审批号:IACUC20170315-07。结果与结论:①3组材料浸提液中小鼠成骨前细胞的线粒体活性与对照组比较差异无显著性意义(P>0.05),3组材料浸提液中人成骨细胞的线粒体活性与对照组比较差异均无显著性意义(P>0.05);3组材料浸提液中,纳米碳化羟基磷灰石海藻酸盐浸提液中两种细胞的线粒体活性最高;②3组材料浸提液对小鼠成骨前细胞与人成骨细胞的毒性均明显低于对照组(P<0.05);3组材料浸提液中,纳米碳化羟基磷灰石海藻酸盐浸提液对两种细胞的毒性最低;③纳米碳化羟基磷灰石海藻酸盐材料植入后7d的RANKL质量浓度低于21d(P<0.001),植入后42d的骨保护素质量浓度高于植入后7,21d(P<0.001);④结果表明相对于羟基磷灰石/β-磷酸三钙与单纯羟基磷灰石材料,纳米碳化羟基磷灰石海藻酸盐材料具�
BACKGROUND: There are many kinds of dental pulp replacement materials, which can be divided into natural biological materials, artificial synthetic materials, and composite materials. Various types of materials can also be synthesized by chemical and biological methods, all of which have their own advantages and disadvantages. OBJECTIVE: To compare cytotoxicity and biological compatibility of three kinds of alveolar bone repair materials including hydroxyapatite/β-tricalcium phosphate, carbonated hydroxyapatite alginate, and pure hydroxyapatite. METHODS: Mouse pre-osteoblasts and human osteoblasts were cultured together with the leaching liquor of three biomaterials respectively. Cell mitochondrial activity was detected by XTT assay (cells cultured separately were used as the control group), and cytotoxicity was detected by crystal violet assay (cells cultured in DMSO were used as the control group).Twenty Wistar rats purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd were used to prepare the alveolar bone defect model of the right maxillary central incisor. Then the alveolar bone defect models were randomly divided into four groups. In the control group, no material was implanted into the bone defect region. In the other three groups, hydroxyapatite/β-tricalcium phosphate, carbonated hydroxyapatite alginate, and pure hydroxyapatite were implanted into the bone defect region. At 7, 21 and 42 days after implantation, serum concentrations of receptor activator for nuclear factor-κB ligand and osteoprotegerin were measured. This study was approved by Animal Ethics Committee, Southwest Medical University, China (approval No. IACUC20170315-07). RESULTS AND CONCLUSION: There was no significant difference in the mitochondrial activity of the mouse preosteoblasts and human osteoblasts between three material groups and the control group (P > 0.05). The mitochondrial activity of mouse preosteoblasts and human osteoblasts was higher in the hydroxyapatite/β-tricalcium phosphate and carbonated hydrox
作者
程扬
刘敏
朱忠焰
高莎莎
Cheng Yang;Liu Min;Zhu Zhongyan;Gao Shasha(Stomatological Hospital Affiliated to Southwest Medical University,Luzhou 646000,Sichuan Province,China;Stomatological Department,Zigong First People's Hospital,Zigong 643000,Sichuan Province,China;Deyang Stomatological Hospital,Deyang 618000,Sichuan Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第30期4804-4810,共7页
Chinese Journal of Tissue Engineering Research
基金
四川省科技厅-泸州市科技局-泸州医学院联合项目(LY-51),项目负责人:刘敏~~
关键词
羟基磷灰石
Β-磷酸三钙
海藻酸盐
组织工程
颌骨缺陷
骨再生
生物支架材料
生物相容性
hydroxyapatite
β-tricalcium phosphate
carbonated hydroxyapatite alginate
tissue engineering
Jaw defects
bone regeneration
biological scaffold material
biocompatibility
