摘要
背景:有文献指出,向聚乳酸中加入生物活性玻璃颗粒可提高材料的力学性能。目的:采用真空冷冻干燥法制备聚乳酸/生物活性玻璃骨组织工程支架材料,并分析其性能。方法:以1,4-二氧六环与二氯甲烷为致孔剂,采用真空冷冻干燥技术制备含生物活性玻璃质量分数分别为10%,20%,30%的聚乳酸/生物活性玻璃复合材料,检测复合材料的孔隙率、抗压强度。将含10%,20%,30%生物活性玻璃复合材料浸泡于模拟体液中2周,观察支架浸泡前后的微观结构与元素变化。分别以聚乳酸浸提液、含10%,20%,30%生物活性玻璃复合材料浸提液与苯酚溶液(阳性对照)培养L929成纤维细胞,以常规培养的细胞为对阴性对照,培养1,3,5d,采用MTT法检测细胞增殖。结果与结论:①含10%,20%生物活性玻璃复合材料的孔隙率高于聚乳酸材料(P<0.05);②各聚乳酸/生物活性玻璃复合材料的抗压强度均高于聚乳酸材料(P<0.05),并且含20%生物活性玻璃复合支架组的抗压强度高于含10%,30%生物活性玻璃复合支架组(P<0.05);③扫描电镜显示,复合材料孔隙内壁上均有大量微孔结构,生物活性玻璃分散在材料中,孔隙大小不均,孔隙之间相互沟通,随着生物活性玻璃含量的增加,孔隙有堵塞现象;在模拟体液中浸泡2周后,复合材料有明显的羟基磷灰石生成,并且钙、磷、硅元素比例升高;④培养1,3,5d,含10%,20%,30%生物活性玻璃复合支架组表面的细胞增殖快于阳性对照组(P<0.05),与阴性对照组、聚乳酸组比较差异无显著性意义(P>0.05);⑤结果表明,采用真空冷冻干燥法制备可制备具有良好孔隙率、抗压强度与细胞相容性的聚乳酸/生物活性玻璃复合支架。
BACKGROUND: Bioactive glass added into polylactic acid can improve the mechanical property of the material. OBJECTIVE: To prepare the tissue-engineered scaffold of polylactic acid/bioactive glass composites by vacuum freeze-drying technique and study its performance. METHODS: For 1,4-dioxane and dichloromethane as pore-forming agents, polylactic acid containing 10%, 20% and 30% bioactive glass were prepared by vacuum freeze-drying technique. The porosity and compressive strength were detected. In order to observe the microstructure and element changes of composites before and after soaked into the simulated body fluid for 2 weeks. L929 fibroblasts were cultured with polylactic acid leach liquor, polylactic acid/bioactive glass composites leaching liquor and phenol solution, and the cells of being cultured in routine culture were used as control, respectively. After culture for 1, 3 and 5 days, MTT was used to detect cell proliferation. RESULTS AND CONCLUSION:(1) The porosity of polylactic acid containing 10% and 20% bioactive glass was higher than that of polylactic acid (P < 0.05).(2) The compressive strength of polylactic acid/bioactive glass composite was higher than that of polylactic acid (P < 0.05), and the compressive strength of 20% bioactive glass composite was higher than that of 10% and 30% bioactive glass composites (P < 0.05).(3) Scanning electron microscopy showed that the inner pore wall of polylactic acid materials has a large amount of micropore structures, bioactive glass distributed in the composite, the pore size was uneven and the pores communicated with each other. As the increasing of bioactive glass contents, there were lots of blocked pores. After immersion in simulated body fluid for 2 weeks, polylactic acid/bioactive glass containing composites showed obvious hydroxyapatite formation, but there was no hydroxyapatite formation in polylactic acid. The immersion of polylactic acid was only the change of PH value, but the ratio of calcium and phosphorus was higher than that before soaking i
作者
马丽娟
邓久鹏
尹浩月
田宜文
Ma Lijuan;Deng Jiupeng;Yin Haoyue;Tian Yiwen(School of Stomatology,North China University of Science and Technology,Tangshan 063000,Hebei Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第30期4793-4798,共6页
Chinese Journal of Tissue Engineering Research
基金
河北省教育厅重点项目(ZD2015016),项目名称:掺锶透钙磷石涂层对骨质疏松种植体骨结合的作用及机制研究,项目参与者:邓久鹏~~
关键词
聚乳酸/生物活性玻璃复合材料
生物玻璃
聚乳酸
冷冻干燥法
骨组织工程支架材料
孔隙率
抗压强度
细胞增殖
polylactic acid/bioactive glass containing composite material
bioactive glass
polylactic acid
vacuum freeze-drying technique
bone tissue-engineered scaffold material
porosity
compressive strength
cell proliferation
