摘要
为给小麦品质性状的标记辅助选择提供备选分子标记,并进一步定位和克隆小麦部分品质相关性状的QTL,本研究利用普通小麦Heyne×Lakin杂交F2代单粒传获得的145个F8代重组自交系(Recombinantinbred line, RIL)群体,构建了含有2 082对标记(1 980对SNP标记和102对SSR标记)、总长度为2 120.13 cM的遗传连锁图谱,并利用该图谱对小麦连续两年的品质性状(蛋白质含量,湿面筋含量,淀粉含量和Zeleny沉降值)进行了 QTL分析。结果表明,共检测出11个QTL,其中,蛋白质含量有2个QTL,位于2A和5A染色体上,可解释的表型变异率6.94% 12.55%;湿面筋含量QTL有1个,位于5A染色体上,可解释的表型变异率8.40%~ 12.96%;淀粉含量QTL有3个,位于2A和5A染色体上,可解释的表型变异率9.78%~11.23%;Zeleny沉降值QTL有5个,位于2A、5A和7D染色体上,可解释的表型变异率4.75% 20.15%。其中5A染色体上存在这些品质性状QTL富集区,同时具有“一因多效”QTL位点。这个区段的发现,为小麦品质育种提供了参考依据。
In order to provide candidate molecular markers for marker-assisted selection of wheat quality traits and further locate and clone QTLs for some quality-related traits in wheat, 145 F8-generation recombinant selfcrosses obtained from single wheat granules of common wheat Heyne ×Lakin hybrid F2 generation were used in this study. Recombinant inbred line(RIL) population, constructed a genetic linkage map containing 2 082 pairs of markers(1 980 pairs of SNP markers and 102 pairs of SSR markers) with a total length of 2 120.13 cM, and using this map for wheat for two consecutive years QTL analysis was performed on quality traits(protein content, wet gluten content, starch content and Zeleny sedimentation value). The results showed that eleven additive QTLs for quality related traits were detected, including two of them for protein content were mapped on chromosomes 2 A and 5 A, explaining 6.94%~12.55% of the phenotypic variances;one QTL for wet gluten content were located on chro-mosomes 5 A, accounting for 8.40%~12.96% of the phenotypic variances;three QTLs for starch content were mapped on chromosomes 2 A and 5 A, explaining the phenotypic variation of 9.78%~11.23%;five QTLs for Zeleny sedimentation value were located on chromosomes 2 A, 5 A and 7 D, accounting for 4.75%~20.15% of the phenotypic variances. Among them, there are QTL enrichment regions of these quality traits on chromosome 5 A, and there is a "multiple effect" QTL locus. The discovery provide reference for wheat quality breeding.
作者
黄梦豪
刘天相
强琴琴
李春莲
王中华
Huang Menghao;Liu Tianxiang;Qiang Qinqin;Li Chunlian;Wang Zhonghua(State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, 712100)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第12期3966-3973,共8页
Molecular Plant Breeding
基金
陕西省科技创新团队计划项目(2014KCT-25)资助
