摘要
目的探寻人附红细胞体感染SD大鼠模型的构建方法.方法通过给6周龄SD大鼠连续3天腹腔注射人附红细胞体分离纯化液(浓度1×10^7个/微升,100微升/只),并观察大鼠体温、精神状态、摄食饮水、皮肤及巩膜有无黄染、脱毛、血涂片瑞氏染色光镜下计数感染率变化对模型进行评价.结果模型组在感染第0、3、5、7、9、11天的感染率分别为0、17.26%±1.33%、37.02%±1.48%、67.37%±2.52%、62.78%±2.34%、61.32%±1.69%.对照组在第0、3、5、7、9、11天的感染率均为0.结论通过给每只SD大鼠连续3天腹腔注射100μl,1×10^7个/微升人附红细胞体分离纯化液的方式可以感染SD大鼠,成功建立人附红细胞体SD大鼠感染模型.
Objective To explore the method of constructing SD rat model of infected human eperythrozoon. Methods By intraperitoneal injection( concentration 1×10^7/jxl, 100|il/rat) of human eperythrozoon separation and purification fluid to 6 months old rats for 3 days, we observed temperature, mental status, feeding and drinking behavior, skin and sclera yellowness or not, hair removal and the changes of Wringt blood smear samples infection rates under the light microscope , so as to evaluate infection models. Results On day 0 , 3 , 5 , 7 , 9 and 11 , infection rates of model group were 0,17. 26%± 1.33%,37. 02%± 1.48%,67. 37%±2. 52%,62. 78%± 2. 34%, 61.32%±1.69%. Conclusion SD rats can be infected by intraperitoneal injection ( concentration 1×10^7/pil, lOOpil/rat) of human eperythrozoon separation and purification fluid for 3 days, the infection model of SD rats infected with human eperythrozoon was successfully established.
作者
白静
陈瑶
郝雷
刘寰宇
Bai Jing;Chen Yao;Hao Lei(Department of Pathophysiology, Inner Mongolia Medical University, Inner Mongolia 010000, China)
出处
《医学研究杂志》
2019年第6期42-45,共4页
Journal of Medical Research
基金
国家自然科学基金资助项目(812600414)
