摘要
评价3株人源干酪乳杆菌的益生性能,为其在食品和保健品等行业中的应用提供理论支持。通过体外测定3株干酪乳杆菌的对模拟肠道的耐受性、黏附性、抑菌性及抗生素敏感性来评价其益生性能。3株干酪乳杆菌,特别是NCU011056,对高盐、酸和胆盐均具有高耐受性,NCU011056在100g/L NaCl、pH 2.0和3 g/L胆盐环境下存活率分别为95.7%、87.2%和80%;3株干酪乳杆菌对Caco-2细胞系黏附能力各异,其中NCU011056黏附能力较强,黏附指数为60.08%;3株干酪乳杆菌对4种常见革兰氏阳性和革兰氏阴性致病菌均具有较强的抑菌能力;3株干酪乳杆菌对青霉素、阿莫西林、头孢曲松、四环素、氯霉素、红霉素、利福平、环丙沙星均敏感。3株人源干酪乳杆菌,特别是NCU011056,具有良好的益生潜力,可考虑后期应用于食品、保健品等行业中。
The aim of this study was to evaluate the probiotic properties of three strains of Lactobacillus casei isolated from infant feces.Their tolerance and adhesion to simulated gastrointestinal tract,antibacterial ability and antibiotic resistance were measured in vitro.It was found that the three strains of L.casei,especially NCU011056,were highly tolerant to high salt,acid and bile salts.The survival rates of NCU011056 were 95.7%,87.2%,and 80%after 4 h treatment with 10%NaCl,pH=2.0,and 0.3%bile salts,respectively.All three strains had different adhesion ability to Caco-2 cell line,among which,NCU011056 had the strongest adhesion ability with an adhesion index of 60.08%.Moreover,all strains had antibacterial activities against four common gram-positive and gram-negative pathogens.Besides,they were all sensitive to penicillin,amoxicillin,ceftriaxone,tetracycline,chloramphenicol,erythromycin,rifampicin and ciprofloxacin.In conclusion,three strains of L.casei,especially NCU011056,have good probiotic potential and can be applied in foods,health care products and some other industries.
作者
赵雪婷
彭珍
胡敏
黄涛
熊涛
ZHAO Xueting;PENG Zhen;HU Min;HUANG Tao;XIONG Tao(State Key Laboratory of Food Science and Technology (Nanchang University),Nanchang 330047,China;School of Food Science & Technology,Nanchang University,Nanchang 330047,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2019年第12期15-19,34,共6页
Food and Fermentation Industries
基金
国家重点研发计划(2017YFD0400503-3)
江西省优势科技创新团队建设计划项目(20181BCB24002)
江西省蔬菜产业技术体系岗位专家项目(JXARS-06)
关键词
干酪乳杆菌
耐受性
黏附性
抑菌性
抗生素耐药性
Lactobacillus casei
tolerance
adhesion
bacteriostatic ability
antibiotic resistance
