摘要
目的:探讨在低氧条件下运动对营养性肥胖大鼠腓肠肌中miR-27/PPARγ及其下游脂代谢相关调控基因和蛋白表达的影响。方法:SD大鼠喂饲高脂饲料构建营养性肥胖大鼠模型,建模成功后随机分为5组,分别为正常氧浓度组(C组)、低氧训练1周组(E1组)、低氧训练2周组(E2组)、低氧训练3周组(E3组)和低氧训练4周组(E4组),每组10只。低氧训练大鼠置于13.6%低氧环境(相当于实际海拔3500m高度氧含量),水平动物跑台训练(20m/min,1h/天,5天/周)。末次训练24h后(禁食12h)麻醉取右侧腓肠肌中端。RT-PCR检测大鼠腓肠肌miR-27、PPARγ、ATGL、CD36mRNA的表达量,WestentBlot检测大鼠腓肠肌PPARγ、ATGL、CD36、蛋白表达量。结果:1)肥胖大鼠腓肠肌miR-27表达量在前2周降低,随后2周又逐渐上升,E4组miR-27相对表达量非常显著高于其他各组(P<0.01)。2)PPARγmRNA随低氧训练时间的增加表达量逐渐上升,但E3组出现下降趋势,与E1组、E2组(P<0.05)和E4组(P<0.01)相比呈显著降低,C组显著低于E4组(P<0.05);PPARγ蛋白表达量随低氧训练时间的延长先下降后上升,C组和E1组均非常显著高于E2、E3、E4组(P<0.01)。E2组显著低于E3组(P<0.05)和E4组(P<0.01)。3)ATGLmRNA表达量逐渐增多,其中C组非常显著低于E1、E2组、E3组、E4组(P<0.01),E3组显著低于E4组(P<0.01);ATGL蛋白相对表达量逐渐增加,C组与E1组均非常显著低于E3组(P<0.01)和E4组(P<0.05)。4)CD36mRNA表达量先下降后上升,C组显著高于E2组、E3组(P<0.05),E1组显著高于E2组(P<0.05);C组CD36蛋白表达量显著低于E2和E3组(P<0.05)。结论:肥胖大鼠腓肠肌中miR-27在低氧训练第4周增加显著,介导PPARγ调控下游脂代谢相关靶基因的表达,从而调控大鼠机体脂代谢水平,这可能是低氧训练减脂降体重的分子学机制。
Objective: To explore the effects of hypoxic exercise on miR-27/PPARγ and lipid metabolism related gene and protein expression levels in gastrocnemius muscle of obese rats. Methods: The high-fat diet induced obese rats were randomly divided into 5 groups(n=10 in each group), i.e., a normal oxygen concentration group(C), a hypoxia-exercises-for-1-week group(E1), a hypoxiaexercises-for-2-weeks group(E2), a hypoxia-exercises-for-3-weeks group(E3) and a hypoxia-exercises-for-4-weeks group(E4). The exercise training was conducted in an environment of 13.6% hypoxic(equivalent to the actual altitude of 3500 meters high oxygen content), rats were running on an animal treadmill in a speed of 20 m/min for 1 h/d,5 d/w. After 24 h of the last exercise(12 h fasting), the middle of the right gastrocnemius muscle was collected. The RT-PCR was used to detect the mRNA levels of miR-27, PPARγ, CD36 and ATGL, the protein levels of PPARγ, CD36 and ATGL were measured by using Western blotting. Results: 1) In the first two weeks, the expression levels of miR-27 was gradually decreased in gastrocnemius muscle, but it was gradually increased in the subsequent two weeks. The expression levels of miR-27 in group E4 were significantly higher than other groups(P<0.01). 2) The mRNA levels of PPARγ were gradually increased during the training session, but the mRNA levels of PPARγ in group E3 were significantly lower than that of group E1 and E2(P<0.05), and group E4(P<0.01);the group C was significantly lower than group E4 as well(P<0.05);the protein levels of PPARγ showed a trend from decline to rise during training session, both group C and E1 were significantly higher than group E2?E3 and E4(P<0.01), the group of E2 was significantly lower than E3 group(P<0.05) and E4 group(P<0.01). 3) The mRNA levels of ATGL in group C were significantly lower than E1?E2?E3 and E4 groups(P<0.01), and the group E3 was significantly lower than group E4(P<0.01);moreover, the protein levels of ATGL were lower in group C and E1 than that of group E3(P<0.
作者
朱磊
路瑛丽
冯连世
张素娴
ZHU Lei;LU Yingli;FENG Lianshi;ZHANG Suxian(Qufu Normal University, Qufu 273165, China;China Institute of Sport Science, Beijing 100061, China;Rizhao Sports School, Rizhao 276800, China)
出处
《体育科学》
CSSCI
北大核心
2019年第6期55-61,共7页
China Sport Science
基金
国家自然科学基金资助项目(31471139)
关键词
低氧训练
miR-27
PPARΓ
肥胖大鼠
脂肪酸代谢
hypoxia exercise
microRNA 27
peroxisome proliferators-activated receptor gamma
obese rat
fatty acids metabolism
