摘要
【目的】探讨半根交替干旱胁迫对大豆农艺性状及籽粒代谢物的影响,为研究大豆品质提升及抗旱代谢调控机理奠定基础。【方法】以两个耐旱性差异显著的大豆品种(耐旱型ND12、敏感型C103)为研究材料,开展室内盆栽试验,考察了不同水分胁迫下大豆的株高、单株荚数、百粒重等农艺性状,并采用高效液相色谱法分析籽粒代谢群体的变化规律。【结果】在半根交替干旱胁迫下,两个大豆品种的外观性状变化存在差异,但并不显著(P>0.05),抗性品种ND12的株高、茎粗、分枝数、主茎节数等指标呈下降趋势,敏感型品种C103却出现了小幅增长;胁迫对大豆产量性状的影响较大,两个品种的百粒重均极显著降低,单株产量显著降低,ND12的降幅低于C103;干旱敏感型大豆籽粒化学成分对胁迫的响应更剧烈,皂苷合成通路及丙酮醛降解通路上调,大豆皂苷和谷胱甘肽含量显著上升;木质素糖苷合成通路及苯丙氨酸合成通路显著上调,松柏醇、芥子醛等含量显著上升。【结论】有望通过适度的半根干旱栽培,在维持大豆正常生长的前提下,提升皂苷、谷胱甘肽等品质性状;通过籽粒代谢群体的变化情况,能够为抗旱性大豆种质资源的筛选提供参考;胁迫环境下大豆籽粒木质化程度的加剧,可能对大豆代际间应对非生物胁迫有重要作用。
【Objective】The objective of the study was to explore the effect of alternating split-root drought stress on the agronomic characters and metabolites of soybean seed,and to provide a reference for quality improvement of soybean seed and mechanism of drought resistance metabolism.【Method】Two soybean cultivars with different drought tolerance named as ND12 (drought-tolerance species) and C103 (drought-sensitive species) were grown under indoor potted conditions. The agronomic traits such as plant height,pod number per plant and 100-grain weight of soybean plants grown under different drought stress condition were investigated,and the regulation of metabolites in soybean seeds was compared by HPLC analysis.【Result】Appearance characters of two soybean cultivars were not significantly differet (P>0.05). Plant height,stem diameter,number of branches and nodes of main stem of ND12 showed a decrease trend under alternating split-root drought stress,while C103 had a slight increase. The stress had a greater impact on soybean yield traits. Both 100-seed weight and yield per plant of two soybean varieties decreased,and the decline magnitude was lower in ND12 than C103. The chemical composition of drought-sensitive soybean seed was more sensitive to the stress. The saponin synthesis pathway and the methylglyoxal degradation pathway in seeds showed an increase trend and soybean saponin and glutathione contents increased significantly.【Conclusion】It is possible to elevate saponin and glutathione under the premise of maintaining the normal growth of soybean through moderate semiroot drought cultivation mode. It can also provide reference for the selection of drought-resistant soybean germplasm resources through the change of seed metabolites.
作者
谢丛薇
张潇文
杨才琼
胡宝予
杨峰
王小春
刘江
杨文钰
张静
XIE Congwei;ZHANG Xiaowen;YANG Caiqiong;HU Baoyu;YANG Feng;WANG Xiaochun;LIU Jiang;YANG Wenyu;ZHANG Jing(Key Laboratory of Crop Ecophysiology and Farming System in Southwest,Ministry of Agriculture,Chengdu 611130,China;Sichuan Engineering Research Center for Crop Strip Intercropping system,Chengdu 611130,China;Institute of Ecological Agriculture,Sichuan Agricultural University,Chengdu 611130,China)
出处
《四川农业大学学报》
CSCD
北大核心
2019年第3期288-295,共8页
Journal of Sichuan Agricultural University
基金
国家重点研发计划(No.2016YFD0300200)
大豆产业技术体系专项(CARS-04-PS19)
国家自然科学基金(No.31301277)
中国博士后科学基金项目(No.2017T100707,2014M560724)
关键词
大豆
分根
干旱胁迫
代谢组
农艺性状
soybean
split-root
drought stress
metabolomics
agronomic characters
