摘要
[目的]本文旨在探讨铅对小鼠精子发生和精子活力的影响及机制。[方法]建立铅胁迫试验小鼠模型;涂片观察精液品质并拍照;制备精子悬液,用穗加精液分析(SSA)自动检测系统测定精子运动学参数及精子活力参数;用荧光定量PCR检测Oct4、DAZ1、SCP3、Tsc21、ACR、Acrv1、cyclinA1、SPATA46和LDH-C4基因的表达水平;观察睾丸组织形态,计数生精小管数和生精细胞数。[结果]与对照组相比,铅胁迫下小鼠活动精子的比例低于50%,精子形态普遍异常;精子曲线运动速度、直线运动速度、平均路径速度、精子头侧摆幅度、鞭打频率、平均角位移与对照组相比均极显著下降(P<0.001),精子运动的直线性、前向性、摆动性与对照组相比无显著差异(P>0.05),精子活力极显著下降(P<0.001);Oct4和DAZ1基因表达水平极显著降低(P<0.001),SCP3、ACR和Acrv1基因表达水平极显著下降(P<0.01),cyclinA1和SPATA46基因表达水平显著降低(P<0.05),而Tsc21和LDH-C4基因表达水平无显著差异(P>0.05)。睾丸生精小管和生精细胞数均极显著减少(P<0.001),部分生精细胞坏死,并在生精细胞坏死集中区域可见支持细胞增多,未见成熟的精子,睾丸间质炎性浸润,间质细胞数明显减少。[结论]铅胁迫可引起睾丸间质细胞损伤和生精障碍。铅可能通过下调Oct4、DAZ1、SCP3和SPATA46基因表达,使精子数量减少,精子成熟停滞,畸形精子数增加,精子活力下降。
[Objectives] The paper aimed to investigate the effect and mechanism of lead on spermatogenesis and sperm motility in mice.[Methods] The mouse model of lead stress test was established,the semen quality was observed and photographed by smear,the sperm suspension was prepared,and the sperm motility parameters and sperm motility parameters were measured by Suiplus semen analysis(SSA)automatic detection system. The expression levels of Oct 4,DAZ1,SCP3,Tsc21,ACR,Acrv1,cyclin A1,SPATA46 and LDH-C 4 genes were detected by fluorescence quantitative PCR. The morphology of testicular tissue was observed,the number of seminiferous tubule and spermatogenic cell were counted.[Results] Compared with the control group,the proportion of mobile sperm under lead stress was significantly lower than 50%,and the sperm morphology of mice was generally abnormal. The sperm curvilinear velocity,straight-line velocity,average path velocity,sperm head swing amplitude,whipping frequency and average angular displacement were significantly lower than those in the control group( P <0.001). There was no significant difference in linear,forward and swing between the treatment group and the control group. Sperm motility decreased significantly( P <0.001). Oct 4 and DAZ1 gene expression levels significantly decreased( P <0.001), SCP 3,ACR and Acrv1 gene expression levels significantly decreased( P < 0.001 ), cyclinA 1 and SPATA46 gene expression levels significantly decreased( P <0.05). However,there was no significant difference in gene expression between Tsc 21 and LDH-C4 . The number of testicular seminiferous tubule and spermatogenic cells decreased significantly ( P <0.001). Some spermatogenic cells were necrotic,and sertoli cells increased in the concentrated area of spermatogenic cell necrosis. There was no mature sperm and testicular interstitial inflammatory infiltration. The number of interstitial cells decreased significantly.[Conclusions] Lead stress can cause damage to Leydig cells and sperm dysfunction. Lead may reduce the number of
作者
杨治河
邹彦
曹言
陈芳慧
李友余
陈婧
王校怡
YANG Zhihe;ZOU Yan;CAO Yan;CHEN Fanghui;LI Youyu;CHEN Jing;WANG Xiaoyi(Department of Anatomy,Chuzhou City Vocation College,Chuzhou 239000,China;College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2019年第4期752-758,共7页
Journal of Nanjing Agricultural University
基金
滁州城市职业学院自然科学重点项目(2018ZK04)
关键词
铅胁迫
精子发生
精液品质
基因表达
精子活力
lead stress
spermatogenesis
semen quality
gene expression
sperm motility
