摘要
微藻是当今代谢工程领域最具潜力的燃料生物质来源之一.其结构简单、基因可操作性强的特点使其可通过不同的代谢工程手段得到丰富多样的生物燃料和高价值产物.本文系统地总结了近年来国内外利用微藻在脂质、氢气、乙烯、醇类、脂肪醇和脂肪烃、糖类、萜类及其他高价值产物的生产中取得的进展,并通过介绍着眼于改变光合作用关键酶体或复合物以提升生物质产量的相关研究进展,分析了生物质合成代谢途径的改变对上游光合作用的潜在影响.结合系统生物学及生物信息学方法筛选高效的微藻株系,改变碳流方向以提升生物质的合成效率,实现高效地同源重组,提高外源基因在微藻内的表达效率是微藻代谢工程亟待解决的问题.本文在此基础上结合近年来各个交叉学科的发展趋势,提出了若干改良微藻代谢的新模式,以期对微藻代谢研究及后续的工程改造有所启示.
Algal biofuel is considered as an alternative to fossil fuels.Due to the structure simplicity and genetic tractability of many microalgae,they are also gradually recognized as an ideal factory for the production of various metabolites of high commercial values.In this mini-review,we attempt to summarize recent development on metabolic engineering of microalgae to explore their metabolic versatility for the production of hydrogen,carbohydrates,terpenes,fatty acids,alcohols,aliphatic alkanes and alkenes.In addition,we also present key challenges facing algal engineering,and propose a few strategies that could leverage the advancement in systems biology and genomics.We foresee a bright future for microalgae studies to advance both research and technology development.
作者
郑艺
王志鹏
蒋振雄
王鹏
尹晟
马新雨
许兵
王鑫
ZHENG Yi;WANG A. ZhiPeng;JIANG ZhenXiong;WANG Peng;YIN Sheng;MA XinYu;XU Bing;WANG Xin(Department of Resources and Environment,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Burn Surgery,The First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510080,China)
出处
《中国科学：生命科学》
CSCD
北大核心
2019年第6期717-726,共10页
Scientia Sinica(Vitae)
关键词
分子生物学
基因工程
微藻
生物燃料
碳流
合成生物学
代谢工程
molecular biology
genetic engineering
microalgae
biofuel
carbon flux
synthetic biology
metabolic engineering
