摘要
目的 探讨大黄?虫丸对颈动脉粥样硬化(CAS)家兔颈动脉狭窄程度、血脂指标及斑块的影响。方法 本实验于2017年11月-2018年1月完成。将40只日本大耳白兔随机分为A、B、C、D、E5组,每组8只,雌雄各4只。采用颈动脉内膜气体干燥损伤法制备CAS模型。A组家兔给予普通饲料喂养,纯净水灌胃;B组家兔模型制备后给予高脂饲料喂养,纯净水灌胃;C组家兔模型制备后给予高脂饲料喂养,阿托伐他汀钙+纯净水灌胃;D组家兔模型制备后给予高脂饲料喂养,大黄?虫丸1.5g·kg^-1·d^-1+纯净水灌胃;E组家兔模型制备后给予高脂饲料喂养,大黄?虫丸3.0g·kg^-1·d^-1+纯净水灌胃;5组家兔均连续灌胃8周。比较5组家兔灌胃8周后颈动脉狭窄程度、血脂指标〔包括三酰甘油(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)及高密度脂蛋白胆固醇(HDL-C)〕及病理形态学。结果 (1)灌胃8周后,B组家兔颈动脉狭窄程度重于A、C、E组(P<0.05)。(2)灌胃8周后,B组家兔血清TG、TC、LDL-C水平高于A、C、E组,血清HDL-C水平低于A、C、E组(P<0.05);D组家兔血清TG水平低于B组,C组家兔血清TC水平低于E组,D组家兔血清HDL-C水平低于E组(P<0.05)。(3)HE染色结果显示,A组和C组家兔光镜下动脉形态较为接近;D组和E组家兔动脉内膜均较A组增厚,但较B组薄;D组家兔相较于E组家兔动脉内膜偏厚,尚有轻微隆起,内皮下间隙增宽,可见迁移的平滑肌细胞。结论 大剂量大黄?虫丸(3.0g·kg^-1·d^-1)能有效减轻CAS家兔颈动脉狭窄程度,与阿托伐他汀治疗效果相似;但其改善血脂代谢、消融斑块的作用较阿托伐他汀钙略差。
Objective To investigate the impact of Dahuang Zhechong pill on degree of carotid stenosis,blood lipid parameters and plaques in rabbits with carotid atherosclerosis(CAS).Methods This experiment was completed from November 2017 to January 2018.A total of 40 Japanese big-ear rabbits were randomly divided into five groups,namely Groups A,B,C,D and E,with eight rabbits(four males and four females)in each group.CAS model was prepared by carotid intima gas drying injury method.Rabbits in Group A were fed with common diet and received gavage of pure water;rabbits in Group B were fed with high-fat diet and received gavage of pure water after preparation of the model;rabbits in Group C were fed with high-fat diet,received gavage of atorvastatin calcium combined with pure water after preparation of the model;rabbits in Group D were fed with high-fat diet,received gavage of low-dose Dahuang Zhechong pill(1.5 g·kg^-1·d^-1)combined with pure water after preparation of the model;rabbits in Group E were fed with high-fat diet,received gavage of high-dose Dahuang Zhechong pill(3.0 g·kg^-1·d^-1)combined with pure water after preparation of the model.Rabbits in the five groups continuously gavaged for 8 weeks.Degree of carotid stenosis,blood lipid parameters(including TG,TC,LDL-C and HDL-C)and pathological morphology were compared in the five groups after 8 weeks of gavage.Results(1)After 8 weeks of gavage,degree of carotid stenosis in Group B was statistically significantly more severe than that in Group A,Group C and Group E(P<0.05).(2)After 8 weeks of gavage,serum levels of TG,TC and LDL-C in Group B were statistically significantly higher than those in Group A,Group C and Group E,while serum HDL-C level in Group B was statistically significantly lower than that in Group A,Group C and Group E(P<0.05);serum TG level of in Group D was statistically significantly lower than that in Group B,serum TC level in group C was statistically significantly lower than that in Group E,and serum HDL-C level in Group D was statistically si
作者
方永军
胡亚莉
范小璇
兰杨
徐泽彪
周锋
胡珍渊
韦鹏方
FANG Yongjun;HU Yali;FAN Xiaoxuan;LAN Yang;XU Zebiao;ZHOU Feng;HU Zhenyuan;WEI Pengfang(The Affiliated Hospital of Shaanxi University of Traditional Chinese Medicine,Xianyang 712000,China;Shaanxi University of Traditional Chinese Medicine,Xianyang 712000,China)
出处
《实用心脑肺血管病杂志》
2019年第5期81-85,共5页
Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease
基金
陕西省社会发展科技攻关项目(2016SF-346)
关键词
动脉粥样硬化
颈动脉狭窄
大黄■虫丸
阿托伐他汀钙
动脉狭窄
血脂
斑块
Atherosclerosis
Carotid stenosis
Dahuang Zhechong pill
Atorvastatin calcium
Arterial stenosis
Blood lipid
Plaque
