摘要
目的评价乳糜化脂肪对移植于裸鼠背部皮下人增生性瘢痕的影响。方法选取18只BALB/c裸鼠;收集解放军总医院第四医学中心烧伤整形科行腹部、大腿外侧部脂肪负压抽吸手术获取的脂肪颗粒细胞及面、颈部手术切除的增生性瘢痕组织;脂肪颗粒细胞经过纳米转换头等操作处理为乳糜化脂肪;无菌条件下将增生性瘢痕组织切割成多块小样本;用眼科剪于各个裸鼠背部各作4个皮肤切口,稍做游离后将前述人增生性瘢痕组织块埋植入切口内,制备增生性瘢痕裸鼠动物模型。18只裸鼠按照随机数字表法分为3组,对照组,曲安奈德组和乳糜化脂肪组,每组6只裸鼠,24块瘢痕组织。移植后1周,对照组于每块移植瘢痕组织块内注射0.9%氯化钠溶液(0.05 mL/g);曲安奈德组注射曲安奈德注射液(0.05 mL/g);乳糜化脂肪组注射乳糜化脂肪(0.05 mL/g);之后每周各注射1次。移植后4周取材,比较移植前人增生性瘢痕组织块重量、移植后4周人增生性瘢痕组织块重量变化百分比;行苏木精-伊红染色和Masson染色,生物显微镜下观察组织形态学变化;比较Masson染色半定量分析阳性面积(天蓝色)占整体视野总面积的百分比。行免疫组织化学染色检测核心蛋白聚糖表达。对数据行单因素方差分析和Dunnett-t检验。结果移植前,3组人增生性瘢痕组织块重量比较,差异无统计学意义(F=26.230,P=0.119),移植后4周,乳糜化脂肪组、曲安奈德组、对照组人增生性瘢痕组织块重量变化百分比分别为(73.2±15.9)%、(79.5±8.6)%、(87.0±14.9)%,比较差异有统计学意义(F=16.680,P=0.042),乳糜化脂肪组分别与对照组、曲安奈德组比较,差异均有统计学意义(t=14.130、1.228,P=0.010、0.099)。组织学观察可见,苏木精-伊红染色与Masson染色整体变化趋势相符:对照组真皮层可见大量胶原纤维及纤维细胞,胶原纤维排列紊乱;曲安奈德组真皮层见粗大胶原纤维,
Objective To evaluate the effect of chyle fat on hypertrophic scar of human transplanted under the back of nude mice.Methods Eighteen BALB/c nude mice were selected.Fat granule cells were collected from the abdominal and later thigh fat suction operations and hypertrophic scar tissue excised from the face and neck in the Department of Burns and Plastic Surgery,Fourth Medical Center of PLA General Hospital.Fat granule cells were processed into nano-transformed heads to make chyle fat.Each human hypertrophic scar tissue were cut into small pieces.Four skin incisions were made on the back of each nude mice with scissors.These specimens were implanted into the backs of the mice with 4 pieces per nude mouse.Eighteen nude mice were divided into 3 groups:control group,triamcinolone acetonide group and chyle fat group according to the random number table method,6 nude mice and 24 scar tissues per group.One week after transplantation,the control group was injected with 0.9%sodium chloride solution into each transplanted scar tissue(0.05 mL/g);triamcinolone acetonide was injected into the triamcinolone acetonide group(0.05 mL/g);chyle fat group was injected with chyle fat(0.05 mL/g);and then injected once a week.Comparison of pre-transplant hypertrophic scar tissue weight and percentage change in weight of hypertrophic scar tissue 4 weeks after transplantation was calculated.After hematoxylin-eosin staining and Masson staining,histomorphological changes under biological microscope were observed.Masson staining semi-quantitative analysis of positive area(sky blue)as a percentage of total field of view was compared.Immunohistochemical staining was performed to detect the expression of decorin.Data were processed with One-way ANOVA and Dunnett-t test.Results Before transplantation,there were no significant differences in the weight of hypertrophic scar tissue among the 3 groups(F=26.230,P=0.119).At 4 weeks after transplantation,the percentage change of weight of hypertrophic scar tissue in the chyle fat group,triamcinolone ac
作者
陈俊男
赖琳英
马奎
黄桢雅
周云超
周桂文
梁黎明
陈敏亮
Chen Junnan;Lai Linying;Ma Kui;Huang Zhenya;Zhou Yunchao;Zhou Guiwen;Liang Liming;Chen Minliang(Department of Burns and Plastic Surgery,Fourth Medical Centre of PLA General Hospital,Beijing 100048,China;Medical School of PLA,Beijing 100085,China;Wound Repair Key Laboratory,PLA General Hospital,Beijing 100048,China;Department of Plastic Surgery,Rocket Force Characteristic Medical Center of PLA,Beijing 100088,China)
出处
《中华损伤与修复杂志（电子版）》
CAS
2019年第3期188-194,共7页
Chinese Journal of Injury Repair and Wound Healing(Electronic Edition)
基金
国家自然科学基金项目(81772085)
关键词
乳糜
脂肪组织
瘢痕
增生
小鼠
成纤维细胞
胶原
Chyle
Adipose tissue
Cicatrix
Hyperplasia
Mice
Fibroblasts
Collagen
