摘要
目的比较纳米脂肪与基质血管成分(stromal vascular fraction, SVF)中细胞含量、成分及活性的差异。方法将12例健康求美者行腹部脂肪抽吸术后的脂肪组织制备成纳米脂肪及SVF的悬液各12份,通过锥虫蓝染色及流式细胞术对两组的细胞数量及成分进行对比;同时将两组细胞于体外培养,取两组第6、13天的细胞行流式细胞术检测表面标志物,并进行油红O及HE染色。结果 12份纳米脂肪悬液和SVF悬液比较结果:细胞的平均数分别为(1.85±0.73)×10^6/ml和(5.57±2.63)×10^6/ml,其差异有统计学意义(P<0.05);脂肪来源干细胞(adipose-derived stem cells, ADSCs)中(CD34^+/CD31^-/CD45^-)的平均数分别为(1.92±0.57)×10^5/ml和(3.07±0.87)×10^5/ml,其差异有统计学意义(P<0.05);内皮细胞(CD34^+/CD31^+/CD45^-)的平均数分别为(5.09±1.06)×10^5/ml和(0.19±0.06)×10^5/ml,其差异有统计学意义(P<0.05)。纳米脂肪悬液和SVF悬液,细胞培养至第6天时,ADSCs(CD73^+/CD90^+/CD31^-/CD45^-)平均数分别为(4.23±1.63)×10^5/ml和(5.11±1.33)×10^5/ml,其差异无统计学意义(P>0.05);第13天时,平均数分别为(7.48±1.57)×10^5/ml和(4.67±0.87)×10^5/ml,其差异有统计学意义(P<0.05);培养至第13天时,油红O染色可见两组细胞内出现红染。结论通过实验发现,纳米脂肪悬液中细胞总数量及ADSCs数量较SVF悬液中的少,但其中内皮细胞数量较多,同时其中的ADSCs通过体外培养与SVF中的相同,可明显增殖,并出现细胞内成脂,这为纳米脂肪在辅助脂肪移植中的应用提供了临床参考。
Objective To compare the cell content, components, activity of nano-fat and SVF. Methods Abdominal lipoaspirates were obtained from 12 healthy women with no systemic diseases, and were prepared into 12 nano-fat and SVF suspension. The cells of two group were examined for surface marker expression and counted for cell numbers, then cultured in vitro. Cultured cells were examined for surface marker expression and histology on the sixth and thirteenth days. Results The results of 12 nano-fat and SVF suspension: mean cell number was(1.85±0.73)×10^6/ml and(5.57±2.63)×10^6/ml respectively(P<0.05);Mean cell number of adipose-derived stromal cells(CD34^+/CD31^-/CD45^-)was(1.92±0.57)×10^5/ml and(3.07±0.87)×10^5/ml respectively(P<0.05);Mean cell number of endothelial cells(CD34^+/CD31^+/CD45^-)was(5.09±1.06)×10^5/ml and(0.19±0.06)×10^5/ml respectively(P<0.05);On the sixth days after culture, mean cell number of adipose-derived stromal cells(CD73^+/CD90^+/CD31^-/CD45^-) was(4.23±1.63)×10^5/ml and(5.11±1.33)×10^5/ml respectively(P>0.05);On the thirteenth days, mean cell number was(7.48±1.57)×10^5/ml and(4.67±0.87)×10^5/ml respectively(P<0.05), and oil red o stain show that lipid aggregate in cells in two groups on the same day. Conclusion The study finds that nano-fat suspension have fewer cell content and adipose-derived stromal cells than SVF suspension, but have more endothelial cells.The adipose-derived stromal cells of two group both can proliferation and adipogenesis in vitro. This study can provide reference for clinical application of nano-fat in Cell-Assisted Lipotransfer.
作者
胡强
韩岩
HU Qiang;HAN Yan(Department of Plastic Surgery,PLA General Hospital,Beijing 100853,China)
出处
《中国美容整形外科杂志》
CAS
2019年第6期331-335,共5页
Chinese Journal of Aesthetic and Plastic Surgery
关键词
自体脂肪移植技术
纳米脂肪
基质血管成分
细胞含量
比较分析
Autologous fat transfer
Nano-fat
Stromal vascular fraction cells
Cell content
Comparative analysis
