摘要
目的探讨Sox2和Nanog在非小细胞肺癌(NSCLC)、癌旁组织及正常肺组织中的表达情况及临床意义。方法应用免疫组化EnVision法检测120例NSCLC患者癌组织、30例癌旁组织和30例正常肺组织中Sox2和Nanog蛋白表达情况。结果(1)Sox2和Nanog在NSCLC中阳性表达率分别为59.2%和48.3%,均显著高于癌旁组织和正常肺组织(均P <0.01);(2)NSCLC中Sox2的表达水平与肿瘤大小和病理类型有关(P <0.05),而Nanog的表达水平与肿瘤大小、淋巴结转移、临床分期及病理类型有关(P <0.05);(3)Sox2和Nanog在肺鳞状细胞癌中的阳性表达均明显高于肺腺癌(81.7%vs. 36.7%和58.3%vs. 38.3%,P <0.05);(4)NSCLC中Sox2与Nanog的阳性表达呈正相关(P <0.05)。结论 Sox2和Nanog蛋白在NSCLC中表达上调且呈正相关,提示它们可能共同参与了NSCLC的发生、发展,有望成为其潜在的治疗靶点。
Objective To investigate the expression of Sox2 and Nanog in non-small cell lung cancer(NSCLC),paracancerous tissue and normal lung tissues and to analyze its clinicopathological significance.Methods Immunohistochemical EnVision method was used to detect the expressions levels of Sox2 and Nanog in120 cases of NSCLC,30 cases of paracancer,and 30 cases of normal tissues. Results(1)The positive expression rates of Sox2 and Nanog in NSCLC were respectively 59.2% and 48.3%,which were significantly higher than those in paracancer and normal tissues(all P < 0.01).(2)The expression of Sox2 in NSCLC was related to tumor size and pathological type(P < 0.05). The expression of Nanog was related to tumor size,lymph node metastasis,clinical stage and pathological type(P < 0.05).(3)The positive expression of Sox2 and Nanog in squamous cell carcinoma was higher than that in adenocarcinoma(81.7% vs. 36.7% and 58.3% vs. 38.3%,P < 0.05).(4)Sox2 expression was positively associated with Nanog(P < 0.05). Conclusion Sox2 and Nanog overexpressed in NSCLC and they are positively correlated,which suggests that Sox2 and Nanog,the potential therapeutic target of NSCLC,may participate in its development.
作者
王永芳
宋姗姗
张春芳
陈昊
WANG Yongfang;SONG Shanshan;ZHANG Chunfang;CHEN Hao(The First People′s Hospital of Lianyungang,Affiliated Hospital of Xuzhou Medical University,Lianyungang 222002,China)
出处
《实用医学杂志》
CAS
北大核心
2019年第11期1730-1734,共5页
The Journal of Practical Medicine
基金
连云港市科技局课题(编号:SH1325)
连云港市卫计委课题(编号:201701)
