摘要
目的评价AlCoCrCuFeTix(x=0,0.25,0.5,1)系列高熵合金细胞毒性。方法对AlCoCrCuFeTix(x=0,0.25,0.5,1)系列高熵合金进行MTT毒性实验评价其细胞毒性;流式细胞术AnnexinⅤ-FITC/PI双染法评价其对L929细胞凋亡的影响;免疫荧光染色检测各组L929细胞Ⅰ型胶原的合成水平来评价该系列材料对细胞增殖的影响。结果随着Ti元素的递增,AlCoCrCuFeTix(x=0,0.25,0.5,1)系列高熵合金细胞毒性增大,凋亡率呈上升趋势,而L929细胞的Ⅰ型胶原蛋白表达水平下降。实验表明AlCoCrCuFe具有良好的细胞相容性,细胞毒性各时间点评级均为1级,且凋亡率及Ⅰ型胶原蛋白表达水平与空白对照组无明显差异。结论AlCoCrCuFeTix系列高熵合金中的AlCoCrCuFe具有良好的细胞相容性,可初步满足医疗器械使用的基本要求。
Objective To evaluate the cytotoxicity of AlCoCrCuFeTix(x=0, 0.25, 0.5,1) series high entropy alloys and explore the effect on apoptosis and type I collagen synthesis in L929 cells by flow cytometry and immunofluorescence. Methods The AlCoCrCuFeTix(x=0, 0.25, 0.5, 1) series of high entropy alloys were tested by MTT test to evaluate the cytotoxicity of series of alloys. Annexin V-FITC/PI staining with flow cytometry was used to evaluate the effect of series of materials on apoptosis of L929 cells. Immunofluorescence staining was used to detect the level of collagen I synthesis to evaluate the effect of materials on cell proliferation. Results The cytotoxicity series of high entropy alloys showed an increasing trend with the increase of Ti elements. At the same time, the proliferation rate of L929 cells decreased, the rate of apoptosis increased and the expression level of type Ⅰ collagen in L929 cells decreased. The metal of AlCoCrCuFe had good cell compatibility, the cytotoxicity was Grade 1 at each time, and there was no statistical difference in the apoptotic rate and type Ⅰ collagen expression level compared with the blank control group. Conclusion AlCoCrCuFe alloy in the series of AlCoCrCuFeTix(x=0, 0.25, 0.5, 1) high entropy alloys has good cell compatibility and can preliminarily meet the basic requirements of the application of dental materials.
作者
肖睿
孙宇
喻望清
焦二龙
汪伟佳
张兆钰
张雪
XIAO Rui;SUN Yu;YU Wangqing;JIAO Erlong;WANG Weijia;ZHANG Zhaoyu;ZHANG Xue(Department of Prosthodontics,Affiliated Stomatological Hospital,Harbin Medical University,Harbin 150000,China)
出处
《口腔医学》
CAS
2019年第6期500-504,共5页
Stomatology
