摘要
将餐厨垃圾中的微生物采用营养琼脂培养基(LA)在温度 55 ℃,相对湿度 50% RH 的条件下培养,根据菌落形态进行筛选分离.经过筛选得到细菌 20 株,测定其对淀粉、蛋白质、油脂和纤维素的分解效果,并根据分解效果选出最优菌株 X13 .对其进行形态学、生理生化以及分子生物学的鉴定并测定生长曲线,最终判定菌株X13 为地衣芽孢杆菌(Bacilluslicheniformis).再使用X13 菌株制成菌制剂处理餐厨垃圾,在小型餐厨垃圾处理器中运行 48h 后,测定餐厨垃圾降解率达到 72.82%.X13 菌株制成的菌制剂能够有效提高餐厨垃圾的降解效率,缩短处理时间,所以该地衣芽孢杆菌可以用于餐厨垃圾处理工业.
The efficient degrading and high-temperature resistance bacteria in kitchen waste were isolated according to the characteristics of domestic kitchen waste. The microorganisms in the kitchen waste were cultured for 48 hours under the conditions of a temperature of 55 ℃ and a relative humidity of 50% RH using a nutrient agar medium (LA), and then they were screened and separated according to the colony morphology. 20 strains of bacteria were obtained, and their decomposition effects of starch, protein, oil and cellulose were determined. X13, which has the best decomposition effect, was selected and identified to be Bacillus licheniformis by morphology, physiology, biochemistry and molecular biology. The microbial agent prepared by X13 strain was used to decompose the kitchen waste. After running for 48 hours in the small kitchen waste disposer, the decomposition of the kitchen waste reached 72.82%. The results showed that the microbial agent could effectively improve the decomposition efficiency of the kitchen waste and shorten the decomposition time. Therefore, the B. licheniformis could be used in the kitchen waste decomposition industry.
作者
万文娟
张赟彬
聂志妍
毛彦佳
许静
喻莹
WAN Wen-juan;ZHANG Yun-bin;NIE Zhi-yan;MAO Yan-jia;XU Jing;YU Ying(School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China;School of Public Health, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China;Shanghai Health Medical College School of Medical Technology, Shanghai, 201318, China;Shanghai Jiehao Logistics Facilities Technology Service Co., Ltd., Shanghai, 201400, China)
出处
《食品与机械》
北大核心
2019年第6期54-58,68,共6页
Food and Machinery
基金
上海市联盟计划(编号:LM201764)
关键词
餐厨垃圾
耐高温细菌
筛选
鉴定
菌制剂
应用
kitchen waste
decomposition bacteria
screening
identification
microbial agents
application
