摘要
目的:建立并验证超高效液相色谱法检测布洛芬在儿童患者血浆中的质量浓度。方法:采用Waters Symmetry C_(18)色谱柱,以0.1%H_3PO_4-甲醇(V∶V=30∶70)作为流动相;检测波长为220 nm;流速为1.0 ml/min;柱温为35℃;进样器温度为4℃;进样量为10μl;内标为萘普生。同时检测10例川崎病患儿服用布洛芬后的血药浓度。结果:血浆中的杂质不干扰布洛芬的检测。布洛芬质量浓度在1～150μg/ml范围内线性关系良好(r=0.999 9)。方法回收率约为96.22%～106.52%,日内、日间精密度良好,RSD均<15%;样品24 h和反复冻融稳定性良好,RSD分别为0.33%～2.15%和0.27%～1.86%。结论:本方法灵敏、稳定且可靠,检测结果准确,可供临床用于患儿布洛芬血药浓度测定及药动学研究。
OBJECTIVE:To establish and validate the ultra-performance liquid chromatography (UPLC) for the determination of serum ibuprofen concentration in children.METHODS:Waters Symmetry C 18 was adopted,the mobile phase was 0.1% H 3PO 4-methanol ( V ∶ V =30 ∶70).The detection wavelength was 220 nm and the flow rate was 1.0 ml/min.The column temperature was 35 ℃ and the injector temperature was 4 ℃.The injection volume was 10 μl,internal standard was naproxen.Meanwhile,the serum concentration of ibuprofen in 10 cases of children with Kawasaki disease was detected.RESULTS:Impurities in the plasma did not interfere with ibuprofen detection.Ibuprofen had a good linear relationship in the range of 1 to 150 μg/ml ( r =0.999 9).The recovery rate was from 96.22% to 106.52%,the precision was good in intraday and interday,the RSD was less than 15%,the sample was stable in 24 h and repeated freeze-thaw,the RSD was from 0.33% to 2.15% and from 0.27% to 1.86%,respectively.CONCLUSIONS:The method is sensitive,stable and reliable,and the detection result is accurate,and can be used for clinical determination of the serum concentration and pharmacokinetic study of ibuprofen in children.
作者
张丹
秦伟
刘慧芳
王晓雪
陈文倩
张相林
ZHANG Dan;QIN Wei;LIU Huifang;WANG Xiaoxue;CHEN Wenqian;ZHANG Xianglin(Dept.of Pharmacy,China-Japan Friendship Hospital,Beijing 100029,China)
出处
《中国医院用药评价与分析》
2019年第5期594-596,共3页
Evaluation and Analysis of Drug-use in Hospitals of China
