摘要
目的研究六君子合旋覆代赭汤对反流性食管炎(reflux esophagitis,RE)大鼠食管Cajal间质细胞(interstitial cells of Cajal,ICC)及干细胞因子(stem cell factor,SCF)/c-kit通道的影响。方法采用幽门半结扎+前胃结扎+大黄灌胃法建立RE脾虚证大鼠模型,造模成功35只Wistar大鼠随机分为模型组、西药组、中药高、中、低剂量组共5组,每组7只,另设正常组10只。西药组予以雷贝拉唑+莫沙比利混悬液灌胃;中药高、中、低剂量3组分别给予六君子合旋覆代赭汤高、中、低剂量灌胃;连续给药14天,1 mL/只,1次/天;正常组以及模型组予以等量生理盐水灌胃。第15天取大鼠下段食管组织,利用透射电镜观察ICC超微结构、 Western blot法检测c-kit及SCF蛋白含量以及RT-PCR法检测c-kit和SCF mRNA相对表达量。结果与模型组比较,各治疗组ICC细胞形态结构改善,与周围细胞之间缝隙连接较紧密。与正常组比较,模型组c-kit、SCF蛋白含量及c-kit mRNA、SCF mRNA相对表达水平显著降低(P<0.01);与模型组比较,各治疗组c-kit、SCF蛋白含量均升高(P<0.05,P<0.01),中药低剂量组c-kit mRNA、SCF mRNA相对表达水平比较,差异无统计学意义(P>0.05),其余各治疗组均较模型组显著升高(P<0.01);中药高、中剂量两组间c-kit、SCF蛋白含量及c-kit mRNA、SCF mRNA相对表达水平比较,差异无统计学意义(P>0.05),但中药高剂量组c-kit、SCF蛋白含量及c-kit mRNA、SCF mRNA相对表达水平明显高于低剂量组(P<0.05,P<0.01)。结论六君子合旋覆代赭汤通过促进c-kit、SCF mRNA及其蛋白的表达,能够使受损的ICC恢复正常。
Objective To observe the effect of Liujunzi(LJZ) combined with Xuanfu Daizhe Decoction(XDD) on interstitial cells of Cajal(ICC) and stem cell factor(SCF)/c-kit signal pathway in reflux esophagitis(RE) rats. Methods Using the ligation of half pylorus combined with anterior gastric ligation and rhubarb gavage to establish the RE Pi-deficiency syndrome(PDS) rat model. Thirty-five successfully modeled Wistar rats were randomly divided into five groups, i.e., the model group, the Western medicine(WM) group, high, medium,and low dose traditional Chinese medicine(TCM) groups, respectively, 7 rats in each group. In addition a normal group was set up(n=10). Rats in the WM group were administered with Rabeprazole and Moxapride suspension by gastrogavage. Rats in the three TCM groups were administered with LJZ combined with XDD at high, medium, and low doses, respectively. All medication lasted for 14 successive days, 1 mL each time, once per day. Equal volume of normal saline was administered to rats in the normal and model groups. At day 15 rats′ lower segment of esophagus were removed. The ultrastructure of ICC was observed by transmission electron microscope. The protein contents of c-kit and SCF were detected by Western blot. And the relative expressions of c-kit and SCF mRNA were detected by RT-PCR. Results Compared with the model group, the morphological structures of ICC cells in each treatment group were improved, and the gap between the cells and the surrounding cells was tight. Compared with the normal group, the contents of c-kit and SCF protein and the relative expression levels of c-kit mRNA and SCF mRNA in the model group were significantly lower(P<0.01). Compared with the model group, the contents of c-kit and SCF protein in each treatment group were increased(P<0.05, P<0.01). There was no significant difference in the relative expression levels of c-kit mRNA or SCF mRNA between the low dose TCM group and the model group(P>0.05). And they were significantly higher in the other treatment groups than in the
作者
陈健海
乔大伟
李玉芳
肖云
姜礼双
崔亚
仲婕
张蕾
孔桂美
卜平
CHEN Jian-hai;QIAO Da-wei;LI Yu-fang;XIAO Yun;JIANG Li-shuang;CUI Ya;ZHONG Jie;ZHANG Lei;KONG Gui-mei;BO Ping(Clinical Medical College of Yangzhou University, Jiangsu ,225000;Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases,Jiangsu ,225000)
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2019年第6期716-722,共7页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助项目(No.81173392)
江苏省普通高校研究生实践创新计划(No.SJLX16-0604)
