摘要
为探讨各阶段山麻鸭卵泡发育水平以及激素分泌调控基因的表达情况,对不同发育等级山麻鸭卵泡壁的形态进行了观察,并检测了CYP19A1和3β-HSD基因的表达量.研究发现,发育过程中颗粒层和膜层细胞数量不断增加,密度和厚度也随着细胞的增殖而增长.对产蛋期山麻鸭卵泡膜层和颗粒层CYP19A1和3β-HSD的表达量进行检测结果表明,卵泡颗粒层的CYP19A1基因主要在等级前阶段表达,3β-HSD基因主要在等级阶段表达,卵泡膜层的CYP19A1和3β-HSD基因则主要都在等级阶段表达.
To investigate the level of follicular development and the expression of hormone secretion regulatory genes in shan partridge duck at different stages, the morphological changes of follicular wall of different developmental grades were observed, and the expression of CYP19A1 and 3beta-HSD was detected. It was found that the number of granular and membranous cells increased during development, density and thickness also increased with cell proliferation. According to the results of the expression of CYP19A1 and 3beta-HSD in the follicular membrane and granular layer of laying ducks, CYP19A1 gene in the granular layer of follicles were mainly expressed at the pre-grade stage, 3beta-HSD genes were mainly expressed at the grade stage, while CYP19A1 and 3beta-HSD genes in the follicular layer were mainly expressed at the grade stage.
作者
杨晨
黄雪冰
刘文俊
劳永聪
陈世健
田允波
黄运茂
YANG Chen;HUANG Xuebing;LIU Wenjun;LAO Yongcong;CHEN Shijian;TIAN Yunbo;HUANG Yunmao(Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou 510225, China;College of Animal Science and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China)
出处
《仲恺农业工程学院学报》
CAS
2019年第2期17-22,共6页
Journal of Zhongkai University of Agriculture and Engineering
基金
国家重点研发计划(2016YFD0500510)
广东省自然科学基金(2018A030313630)
广州市科技计划(201804010412)
广东省科技计划(2017A020208069)资助项目
