摘要
目的探讨转化生长因子-β1(TGF-β1)和E-钙黏蛋白(E-cad)的表达与胚胎停育的关系。方法 30例胚胎停育患者为实验组,30例正常妊娠者为对照组。采用实时荧光定量逆转录聚合酶链式反应(RT-PCR)及免疫组化(IHC)检测2组绒毛组织中TGF-β1和E-Cad的表达。结果胚胎停育组TGF-β1 mRNA表达量(0.824±0.26)明显低于对照组(1.316±0.40)(t=2.859,P<0.01);胚胎停育组E-Cad mRNA表达量(1.875±0.51)明显高于对照组(1.001±0.41)(t=6.024,P<0.01)。TGF-β1在胚胎停育组中的阳性表达率(33.3%)显著低于对照组(70.0%);E-Cad在胚胎停育组中的阳性表达率(50.0%)显著高于对照组(30.0%)。结论 TGF-β1的低表达和E-Cad的高表达参与了胚胎停育的发病过程;且TGF-β1的下调可能通过上调E-Cad的表达水平,参与了胚胎停止发育的发生机制。
Objective To study the relationship of transforming growth factor-beta 1(TGF-1) and E-cadherin(E-cad) with early embryonic death. Methods 30 cases of early embryonic death were assigned as study group and 30 cases of early normal embryo development as control group, using quantitative reverse transcript polymerase chain reaction(qRT-PCR) and immunohistochemistry technique(IHC). Results The mRNA expression of TGF-1 in study group was significantly lower than control group(1.316±0.40)(t=2.859, P<0.01). The mRNA expression of E-Cad in study group(1.875±0.51) was significantly higher than control group(1.001±0.41)(t=6.024, P<0.01). The protein expression of TGF-β1 positive rate of the chorionic villus tissues of early embryonic death(33.3%) was significantly lower than the chorionic villus tissues of early normal embryo development(70.0%). The protein expression of E-Cad positive rate of the chorionic villus tissues of early embryonic death(50.0%) was significantly higher than the chorionic villus tissues of early normal embryo development(30.0%). Conclusion The high expression of TGF-β1 and the low expression of E-Cad in the chorionic villus tissues of early embryonic death may play an important role in the process of early embryo death. The over-expression of TGF-β1 may involve in early embryonic death by up-regulating the expression of E-Cad.
作者
王冬亮
胡滨
刘玉玲
WANG Dong-liang;HU Bin;LIU Yu-ling(Department of Obstetrics and Gynecology, the Second Affiliated Hospital of Zhengzhou Univresity, Zhengzhou, Henan 450000, China)
出处
《中国卫生检验杂志》
CAS
2019年第11期1372-1374,1378,共4页
Chinese Journal of Health Laboratory Technology
