摘要
目的:观察穴位贴敷对过敏性鼻炎(AR)大鼠血清免疫球蛋白E(IgE)、转化生长因子beta 1(TGF-β1)和鼻黏膜嗜酸性粒细胞(EOS)的影响,探讨穴位贴敷对AR的作用机制。方法:Wistar大鼠随机分为正常对照组、模型组、药物组和穴贴组,每组10只。采用卵白蛋白注射及鼻黏膜刺激法建立AR大鼠模型。穴贴组大鼠予双侧“肺俞”“脾俞”“肾俞”穴位贴敷,隔日1次,药物组每日予丙酸氟替卡松鼻喷雾剂滴鼻,均治疗14d。观察大鼠治疗前后行为学评分,酶联免疫吸附法检测大鼠血清中IgE、TGF-β1的含量,苏木素伊红染色观察大鼠鼻黏膜EOS浸润情况。结果:与正常对照组比较,模型组大鼠行为学评分及血清IgE、TGF-β1水平明显上升(P<0.05),鼻黏膜EOS数量明显增加;与模型组比较,药物组和穴贴组大鼠行为学评分及血清IgE、TGF-β1水平均明显降低(P<0.05),鼻黏膜无明显EOS浸润;穴贴组与药物组比较,各指标差异无统计学意义(P>0.05)。结论:穴位贴敷对AR的治疗效果显著,其对AR的调节作用可能是通过下调血清中IgE、TGF-β1水平而实现的。
Objective To observe the effect of acupoint application of herbal paste on symptoms of allergic rhinitis(AR),serum immunoglobulin E(IgE)and transforming growth factor beta 1(TGF-β1)level,and number of nasal eosinophils(EOS)in rats with AR,so as to explore its underlying mechanisms.Methods Forty male Wistar rats were randomly divided into normal control,model,medication and acupoint application groups(n=10 rats per group).The AR model was established by intraperitoneal(i.p.)injection of mixture solution of ovalbumin,aluminum hydroxide and normal saline(once every other day,for7 times),and nasal drip plus spray inhalation of ovalbumin(on the following day of i.p.,once daily for 9 days).For acupoint application,the prepared herbal paste(containing White Mustard Seed,Rhizoma Corydalis,unprocessed Radix Kansui,Herba Asari and ginger juice)was applied to bilateral"Feishu"(BL13),"Pishu"(BL20)and"Shenshu"(BL23)for 2 h,once every other day for 7 times.The rats in the medication group were given Fluticasone Propionate nasal spray daily for 14 days.Scores of nasal itching,sneezing and nasal discharge on the day after modeling and the ending of the intervention were used to evaluate behavioral changes.Enzyme linked immunosorbent assay(ELISA)was used to detect the levels of serum IgE and TGF-β1,and the infiltration state of EOS in the nasal mucosa tissue was observed under light microscope after HE staining.Results After modeling and compared with the normal control group,the behavioral scores and the levels of serum IgE and TGF-β1 were significantly higher(P<0.05),and the infiltration state of EOS got worse.Compared with the model group,the increased behavioral score and serum IgE and TGF-β1 levels were evidently suppressed(P<0.05)and EOS infiltration severity in the nasal mucosa was obviously milder in both medication and acupoint application groups.No significant differences were found between the medication and acupoint application groups in behavioral score and serum IgE and TGF-β1 levels(P>0.05).Conclusion Acupoint
作者
陈姗
金禹彤
朱正阳
吴凌韬
宣丽华
CHEN Shan;JIN Yu-tong;ZHU Zheng-yang;WU Ling-tao;XUAN Li-hua(Department of Acupuncture and Moxibustion,The First Affiliated Hospital of Zhejiang University of Chinese Medicine, Hangzhou 310006, China;The First School of Clinical Medicine of Zhejiang University of Chinese Medicine, Hangzhou 310053)
出处
《针刺研究》
CAS
CSCD
北大核心
2019年第6期430-433,共4页
Acupuncture Research
基金
浙江省重大科技专项计划资助项目(No.2014C03046-2)
