摘要
[目的]为获得能够高效降解园林废弃物的真菌并研究其降解能力,从经过堆肥处理的园林废弃物中分离和筛选出1株木质素降解能力较强的真菌。[方法]经过形态分析、ITS分子序列分析,构建系统发育树,鉴定出该株木质素降解真菌种属,并利用该菌株进行固态发酵实验,研究其在固态发酵过程中漆酶、锰过氧化物酶的酶活力变化及其对不同园林废弃物中木质素的降解能力。[结果]从经过堆肥处理的园林废弃物中共挑选出真菌18株,筛选出1株高效的木质素降解真菌,编号为菌株No.11,经鉴定该菌株为构巢曲霉(Aspergillus nidulans(Eidam) G.Winter)。以冬青卫矛叶、冬青卫矛枝、圆柏叶、圆柏枝、连翘枝叶为底物的模拟固态发酵的漆酶酶活力峰值分别为388.7、326.3、461.4、342.7、588.5U·L^-1,锰过氧化物酶酶活力峰值分别为138.3、121.1、104.6、128.6、73.3U·L^-1。添加菌株No.11使冬青卫矛叶、冬青卫矛枝、圆柏叶、圆柏枝、连翘枝叶40d后木质素降解率分别提高了20.40%、22.44%、29.90%、25.28%、15.77%。[结论]筛选出的构巢曲霉对北京地区常见园林废弃物冬青卫矛、圆柏和连翘枝叶中的木质素具有较好的降解能力,在降解园林废弃物方面可能更具有优势。
[Objective] To obtain a fungus that can efficiently degrade landscaping waste,to study its ability to decomposite various landscaping waste,and to select the fungus strain from the composted landscaping waste by a series of isolation and culture.[Method] The species of lignin decomposition strain were identified by morphological analysis,ITS molecular sequence analysis and phylogenetic tree.Solid-state fermentation experiments were carried out for studying the changes of enzyme activity of laccase (Lac) and manganese peroxidase (MnP) of the strain and their ability to decompose lignin in landscaping waste.[Result] A total of 18 fungus were selected from the composted landscaping waste and a highly efficient lignin-decomposing fungus was selected.The strain was designated as strain No.11,and was identified as Aspergillus nidulans (Eidam) G.Winter by morphological analysis and ITS molecular sequence analysis.The enzyme activity peaks of laccase and manganese peroxidase in solid canisters were determined using Japanese spindle leaves,Japanese spindle branches,juniper leaves,juniper branches and forsythia as the substrates were 388.7 and 138.3,326.3 and 121.1,461.4 and 104.6,342.7 and 128.6,588.5 and 73.3 U·L^-1.The addition of strain 11 increased the lignin decomposition rate of Japanese spindle leaves,Japanese spindle branches,juniper leaves,juniper branches and forsythia after 40 days,respectively,by 20.40%,22.44%,29.90%,25.28%,and 15.77%.[Conclusion] The lignin-decomposing fungus,Aspergillus nidulans (Eidam) G.Winter,has a strong ability to decompose the lignin in the branches and leaves of Japanese spindle,juniper and forsythia common to the landscaping waste in Beijing.Aspergillus nidulans (Eidam) G.Winter may have advantages in decomposing landscaping waste.
作者
康跃
李素艳
孙向阳
龚小强
余克非
蔡琳琳
王琳
KANG Yue;LI Su-yan;SUN Xiang-yang;GONG Xiao-qiang;YU Ke-fei;CAI Lin-lin;WANG Lin(College of Forestry,Beijing Forestry University,Beijing 100083,China)
出处
《林业科学研究》
CSCD
北大核心
2019年第3期80-87,共8页
Forest Research
基金
林业公益性行业科研专项(201504205)
关键词
园林废弃物
木质素降解真菌
酶活力
固态发酵
landscaping waste
lignin-degradation fungi
enzyme activity
solid-state fermentation
