摘要
目的探讨促红细胞生成素(EPO)对大鼠肝脏缺血再灌注后肠损伤的保护作用。方法将30只健康成年雄性SD大鼠分为5组(n=6),分别为假手术组(Sham组),缺血再灌注组(I/R组)和促红细胞生成素低剂量组(L组)、中剂量组(M组)和高剂量组(H组)。用夹闭肝左、中叶门静脉和肝动脉的方法制作肝缺血再灌注模型,用HE染色法观察小肠组织的形态学结构,用免疫组织化学染色及Westernblot法观察比较各组基质金属蛋白酶-9(MMP-9)、半胱氨酸水解酶-1(caspase-1)的表达。结果I/R组小肠绒毛结构破坏,部分绒毛断裂脱落,上皮细胞变性坏死,大量炎性细胞浸润。EPO预处理绒毛结构破坏、炎性细胞浸润的程度明显减轻。免疫组织化学染色及Westernblot检测结果显示,与Sham组相比,I/R组小肠组织MMP-9和caspase-1表达水平均上升(P<0.05);与I/R组比较,EPO预处理组MMP-9和caspase-1表达的表达下降,M组较L、H组更低(P<0.05)。结论EPO对肝缺血再灌注后肠损伤的保护作用可能与抑制MMP-9、caspase-1介导的炎性反应有关。
Objective To investigate the protective effect of erythropoietin (EPO) on intestinal injury after hepatic ischemia reperfusion in rats.Methods Thirty healthy male SD rats were divided into 5 groups( n = 6):the Sham group,the ischemia reperfusion group (group I/R),the low-dose-EPO group (group L),the middle-dose-EPO group (group M) and the high-dose-EPO group (group H).The hepatic ischemia reperfusion model was established by occluding the afferent vessels of left and middle lobes and hepatic artery.The morphological structures of small intestine were observed by hematoxylin-eosin(HE)staining.The protein levels of MMP-9 and caspase-1 in liver tissues were detected by immunohistochemical staining and Western blot.Results In group I/R,the structure of intestinal villis was destroyed,some villi were broken off and shed,epithelial cells were degenerated and necrotic,and infiltration of inflammatory cells appeared.The destruction of villi structure and infiltration of inflammatory cells were significantly alleviated by EPO pretreatment.Immunohistochemistry and Western blot results showed that compared with the Sham group,the expression of MMP-9 and caspase-1 increased in group I/R ( P <0.05).Compared with group I/R,the expression of MMP-9 and caspase-1 in the EPO pretreatment group was lower,which was more lower in group M than that in group L and H ( P < 0.05).Conclusion The protection of EPO on intestinal injury after hepatic ischemia reperfusion in rats may be related to the inhibition on the inflammation mediated by MMP-9 and caspase-1.
作者
吴鹏俐
石清明
阳德飞
刘富春
陈晓琴
王静
肖莉
WU Pengli;SHI Qingming;YANG Defei;LIU Fuchun;CHEN Xiaoqin;WANG Jing;XIAO Li(Undergraduate of Anesthesiology,Chengdu Medical College,Chengdu,Sichuan 610500,China;Center for Disease Control and Prevention of Western Theater,Chengdu,Sichuan 610021,China;Undergraduate of Clinical Medicine,Chengdu Medical College,Chengdu,Sichuan 610500,China;Department of Pharmacy,the 324th Hospital of People′s Liberation Army,Chongqing 400020,China;Undergraduate of Medical Imaging,Chengdu Medical College,Chengdu,Sichuan 610500,China;Department of Human Anatomy,Chengdu Medical College,Chengdu,Sichuan 610500,China)
出处
《重庆医学》
CAS
2019年第12期1981-1985,共5页
Chongqing medicine
基金
2017四川省大学生创新实验项目(201713705076)
2016四川省大学生创新实验项目(201613705033)
四川省教育厅项目(18ZB0179)
发育与再生四川省重点实验室项目(SYS16-010)
