摘要
目的探讨一种新型的包裹葡萄糖氧化酶(GOD)/超顺磁性四氧化三铁纳米粒(Fe3O4Nps)的聚乳酸-羟基乙酸共聚物(PLGA)纳米粒子对视网膜母细胞瘤(RB)体外治疗的效果。方法利用双乳化法制备包裹GOD/Fe3O4的PLGA纳米粒子,对其粒径大小、电位、外部形态和内部结构等相关表征进行检测。依据包含的不同纳米粒子设立空白对照组、Fe3O4/PLGA(PF)组、GOD/PLGA(PG)组、GOD/Fe3O4/PLGA(PFG)组;设立不同浓度(0、0.0625、0.125、0.25μg/ml)的PFG纳米粒子组,以验证其毒性作用。检测不同纳米粒子组与Y79细胞共孵育后对Y79细胞的毒性作用、存活情况及活性氧产生情况。结果成功制备出包裹了GOD/Fe3O4的PLGA纳米粒子,其平均粒径为299.3 nm,形态均一呈圆形。纳米粒子与Y79细胞共孵育后被Y79细胞吞噬,产生大量活性氧;细胞毒性实验结果显示,PFG组Y79细胞存活率[(53.648±2.565)%]明显低于空白对照组[(100.028±4.491)%]、PF组[(97.782±17.520)%]、PG组[(87.438±3.537)%](F=21.226,P<0.01);0.25μg/ml的PFG纳米粒子组Y79细胞存活率[(51.770±1.529)%]明显低于对照组[(100.000±5.021)%]、0.0625μg/ml组[(85.723±6.903)%]、0.125μg/ml组[(74.535±8.282)%](F=34.593,P<0.01)。激光共聚焦显微镜下观察,结果显示PFG组Y79细胞大量死亡。结论成功制备了包裹GOD/Fe3O4的新型PLGA纳米粒子;该纳米粒子具有良好的活性氧产生能力,能杀死Y79肿瘤细胞,有望成为一种治疗RB的新方法。
Objective To evaluate the therapeutic potential of a novel type of Poly(lactic-co-glycolic acid)(PLGA)nanoparticles loaded with glucose oxidase(GOD)/superparamagnetic iron oxide nanoparticles(Fe3O4 Nps) on retinoblastoma(RB)cells in vitro. Methods PLGA nanoparticles loaded with GOD/Fe3O4(PFG) were prepared by double emulsification. Their particle size, potential, external morphology, and internal structure were examined. Particles that made of PLGA(control), PLGA loaded with GOD(PG), and PLGA loaded with Fe3O4(PF) are served as control. Y79 cells that were incubated with different particles are termed control group, PF group, PG group, and PFG group. After co-incubation with nanoparticles, cell viability, and reactive oxygen species production were detected. Results PLGA nanoparticles loaded with GOD/Fe3O4 were successfully prepared. The form of PLGA nanoparticles was uniform and showed a round shape with a diameter of 299.3 nm. The nanoparticles were engulfed by Y79 cells after coincubation with Y79 cells, producing a large number of reactive oxygen species. Cytotoxicity test results showed that the cell viability of Y79 cells in PLGA nanoparticle group coated with GOD/Fe3O4 [(53.648±2.565)%] was significantly lower than that in control group[(100.028±4.491)%], PF group [(97.782±17.520)%] or PG group [(87.438±3.537)%](F=21.226, P<0.01);The cell viability of Y79 cells in 0.25 μg/ml PFG nanoparticle group [(51.770±1.529)%] was significantly lower than that in control group [(100.000±5.021)%],0.0625 μg/ml group [(85.723±6.903)%] and 0.125 μg/ml group [(74.535±8.282)%](F=34.593, P<0.05). Massive cell death was detected in the PFG group under laser confocal microscope. Conclusions The novel type of PLGA nanoparticles loaded with GOD/Fe3O4 toxic to Y79 tumor cells with a good reactive oxygen generation ability. It provides a potential treatment for RB.
作者
全鲁亚
乔斌
王志刚
张屿
杜之渝
QUAN Lu-ya;QIAO Bin;WANG Zhi-gang;ZHANG Yu;DU Zhi-yu(Department of Ophthalmology,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China;Chongqing Key Laboratory of Ultrasound Molecular Imaging,Chongqing Medical University, Chongqing 400010, China;Department of Ophthalmology, Chongqing Mingda Ophthalmology, Chongqing 400010, China)
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2019年第5期382-387,共6页
Medical Journal of Chinese People's Liberation Army
基金
重庆市科学技术委员会基础与前沿项目(cstc2018jcyjAX0562)~~
