摘要
基于高效液相色谱-串联质谱系统建立了食品中水产品过敏原的快速筛查和定量检测方法。样品经蛋白质提取、纯化、胰蛋白酶解后,利用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱(UPLC-Q/Exactive-HRMS)结合ProteinPilot软件,基于母离子和碎片离子谱分析,实现蛋白质和多肽的鉴定。再通过基本局部比对搜索工具(BLAST)与Uniprot数据库对比分析,筛选出南美白虾、大闸蟹、青蟹、金枪鱼、大西洋鲑鱼的7种过敏原蛋白的30个特征肽。利用高效液相色谱-三重四极杆质谱(UPLC-QqQ-MS)系统对特征肽进行验证和多反应监测(MRM)定量研究。结果表明,该方法在5~250 mg/kg 范围内线性关系良好,检出限为2~3.5 mg/kg ,平均回收率为88.7%~110.2%。该方法重现性好,通量高,可应用于肉制品和调味料中7种过敏原的快速筛查和定量分析。
A liquid chromatography-tandem mass spectrometry method for the identification of marker peptides of aquatic product allergens and quantitative detection of multiple allergens in meat products and seasonings was developed. The samples were prepared by protein extraction, protein purification, and trypsin hydrolysis. The proteins and peptides were identified using ProteinPilot by the data analysis of the ion spectrum of polypeptide fragments using ultra-performance liquid chromatography-quadrupole/electrostatic orbitrap high-resolution mass spectrometry (UPLC-Q/Exactive-HRMS). The identification of 30 species-specific marker [JP3] peptides in Penaeus vannamei, Eriocheir, Scylla serrata, Thunnus thynnus , and Atlantic salmon by comparison of the basic local alignment search tool (BLAST) with the UniProt database was achieved. The verification and multiple reaction monitoring (MRM) quantitative studies of these marker peptides were performed using a triple quadrupole mass spectrometry (UPLC-QqQ-MS) system. The proposed method showed a good linear relationship in the range of 5-250 mg/kg . The limits of quantitation and observed recoveries were in the range of 2-3.5 mg/kg and 88.7%-110.2%, respectively. This method presents various advantages such as good repeatability and high throughput, suitability for rapid screening, and quantitative analysis of seven aquatic allergens in meat products and seasonings.
作者
孟佳
古淑青
方真
钮冰
邓晓军
郭德华
朱坚
韩芳
MENG Jia;GU Shuqing;FANG Zhen;NIU Bing;DENG Xiaojun;GUO Dehua;ZHU Jian;HAN Fang(Department of Food Engineering, School of Life Science, Shanghai University, Shanghai 200444, China;Technical Center for Animal Plant and Food Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200135, China;Technical Center for Inspection and Quarantine, Anhui Entry-Exit Inspection and Quarantine Bureau, Hefei 230022, China)
出处
《色谱》
CAS
CSCD
北大核心
2019年第7期712-722,共11页
Chinese Journal of Chromatography
基金
国家重点研发计划项目(2018YFC1603603)
上海市中央引导地方科技发展专项项目(YDZX20173100004528)
上海市技术标准专项项目(17DZ2201100)
长三角科技合作项目(17395810102,1804b06020349)~~
关键词
液相色谱-串联质谱
特征肽段
水产品过敏原
肉制品
调味料
liquid chromatography-tandem mass spectrometry (LC-MS/MS)
marker peptides
aquatic product allergen
meat products
seasoning
