摘要
目的 观察miR-125b在食管鳞癌组织和不同分化程度的食管鳞癌细胞中的表达变化,并分析其与食管鳞癌患者临床病理参数及预后的相关性。方法 体外培养不同分化程度的食管鳞癌细胞系(KYSE410、TE-13、EC9706、KYSE510)和人正常食管细胞系Het-1A,收集 89例手术切除的食管鳞癌和癌旁组织标本及其患者的临床和随访资料。采用qRT-PCR检测上述组织和细胞中miR-125b的表达;分析组织标本中miR-125b表达与患者临床病理参数及预后的相关性。结果 体外培养的食管鳞癌细胞中miR-125b表达显著低于正常食管细胞( P <0.05),且低分化食管鳞癌细胞(KYSE410、TE-13)中miR-125b表达显著低于高分化食管鳞癌细胞(EC9706、KYSE510)( P <0.05)。食管鳞癌组织中miR-125b表达显著低于癌旁组织( P <0.05),且其miR-125b低表达与pTNM分期、淋巴结转移及肿瘤分化程度相关( P <0.05)。生存分析显示miR-125b低表达患者的预后较差﹙ P <0.05﹚,Cox回归分析显示miR-125b低表达是影响食管鳞癌患者预后的独立影响因子( P <0.05)。结论 miR-125b的异常低表达与食管鳞癌的发生和进展密切相关,是影响患者预后的独立危险因素。
Objective To detect the expression of miR-125b in esophageal squamous cell carcinoma (ESCC) tissues and differentiated ESCC cells, and to analyze its correlation with clinicopathological parameters and prognosis of ESCC patients. Methods ESCC cell lines including KYSE410, TE-13, EC9706, KYSE510 and human normal esophageal cell lines, Het-1A were cultured in vitro. The clinical data of 89 cases of esophageal squamous cell carcinoma and adjacent tissues were collected. The expression of miR-125b in the above tissues and cells was detected by qRT-PCR. The clinicopathological parameters and follow-up data of ESCC patients were collected, and the correlation between the expression of miR-125b in tissue samples and clinicopathological parameters and prognosis was analyzed. Results The expression of miR-125b in ESCC cells was significantly lower than that in human normal esophageal cells ( P <0.05), and the expression of miR-125b in low-differentiated ESCC cells (KYSE410, TE-13) was significantly lower than that in highly differentiated ESCC cells (EC9706, KYSE510)( P <0.05). The expression of miR-125b in ESCC tissues was also significantly lower than that in adjacent tissues ( P <0.05), and the low expression of miR-125b was closely related to pTNM, lymph node metastasis and the degree of differentiation of tumors ( P <0.05). Survival analysis showed that the prognosis of patients with low expression of miR-125b was poor ( P <0.05). Cox regression analysis showed that low expression of miR-125b was an independent prognostic factor in patients with ESCC ( P <0.05). Conclusions The abnormal low expression of miR-125b is closely related to the occurrence and progression of ESCC, and is an independent risk factor affecting the prognosis of patients.
作者
方玉松
刘峻岭
张志平
王宗明
尹立国
许鹏
Fang Yusong;Liu Junling;Zhang Zhiping;Wang Zongming;Yin Liguo;Xu Peng(Department of Thoracic and Esophageal Surgery,Jinan Central Hospital Affiliated to Shandong University,Jinan 250013,China;Department of Operative Anesthesiology,Jinan Central Hospital Affiliated to Shandong University,Jinan 250013,China)
出处
《中华胸部外科电子杂志》
2019年第2期106-110,共5页
CHINESE JOURNAL OF THORACIC SURGERY：Electronic Edition
关键词
食管肿瘤
鳞癌
miR-125b
Esophageal neoplasms
Squamous cell carcinoma
miR-125b
