摘要
目的探讨急性脑梗死(ACI)患者血清microRNA-124(miR-124)和microRNA-182(miR-182)的表达水平,及二者联合检测对脑梗死诊断及预后评估的价值。方法选取ACI患者120例作为观察组,同期健康体检者80例作为对照组。观察组患者根据脑部梗死体积进一步分为小梗死组(<5cm^3)、中梗死组(≥5cm^3且≤10cm^3)和大梗死组(>10cm^3)。检测所有入组患者血清miR-124和miR-182的相对表达量,通过受试者工作特征曲线(ROC曲线)分析血清miR-124和miR-182以及二者联合诊断ACI的价值。ACI患者随访1年,分析血清miR-124和miR-182与预后的相关性。结果观察组患者血清miR-124和miR-182的相对表达量分别为(2.63±0.59)和(2.69±0.69),显著高于对照组[分别为(1.08±0.32)和(1.07±0.46)](P<0.05)。中梗死组和大梗死组miR-124相对表达量显著低于小梗死组(P<0.05),而miR-182相对表达量显著高于小梗死组(P<0.05)。Pearson相关分析显示,miR-124相对表达量与脑部梗死体积呈负相关(r=-0.613,P<0.01),miR-182相对表达量与脑部梗死体积呈正相关(r=0.761,P<0.01)。miR-124以1.34为截断值时诊断ACI的灵敏度为73.33%,特异度为90.00%,ROC曲线下面积为0.775(95%CI0.715~0.834,P=0.030);miR-182以1.45为截断值时诊断ACI的灵敏度为66.67%,特异度为87.50%,ROC曲线下面积为0.675(95%CI0.602~0.749,P=0.038);二者联合诊断的灵敏度为88.33%,特异度为86.25%,ROC曲线下面积为0.811(95%CI0.756~0.866,P=0.028)。联合检测ROC曲线下面积显著高于单项检测(P<0.05)。随访预后发现,联合诊断为阳性的患者在治疗后8个月、12个月的死亡率显著高于联合诊断为阴性的患者(P<0.05)。结论miR-124与miR-182在ACI患者血清中呈高水平表达,二者联合检测对ACI的诊断及预后评估有较好的价值。
Aim To investigate the expression levels of serum microRNA-124 (miR-124) and microRNA-182 (miR-182) in acute cerebral infarction (ACI) and the value in the combined diagnosis and prognosis of ACI.Methods 120 patients with ACI were enrolled as the observation group,and 80 healthy subjects were used as healthy controls.According to the brain infarct volume,patients of the observation group were divided into small infarction group(<5 cm^3),middle infarction group (≥5 cm^3 and ≤10 cm^3) and large infarction group (>10 cm^3).The relative expression levels of serum miR-124 and miR-182 were measured in all enrolled patients,and the value of combined diagnosis was analyzed by receiver operating characteristic curve (ROC curve).ACI patients were followed up for one year,and the prognosis of the combined positive and negative groups was analyzed.Results The relative expressions of serum miR-124 and miR-182 in ACI patients were (2.63±0.59) and (2.69±0.69),respectively,which were significantly higher than those in the control group((1.08±0.32) and (1.07±0.46))(P<0.05).The relative expression of miR-124 in the middle and large infarction group was significantly lower than that in the small infarction group (P<0.05).The relative expression of miR-182 in the middle and large infarction group was significantly higher than that in the small infarction group (P<0.05).Pearson correlation analysis showed that the relative expression of miR-124 was negatively correlated with brain infarct volume (r=-0.613,P<0.01),and the relative expression of miR-182 was positively correlated with brain infarct volume (r=0.761,P<0.01).When miR-124 was cut off by 1.34,the sensitivity of diagnosis of ACI was 73.33%,the specificity was 90.00%,the area under the curve was 0.775 (95%CI 0.715~0.834,P=0.030);when miR-182 was cut off by 1.45,the sensitivity of diagnosis of ACI was 66.67%,the specificity was 87.50%,the area under the curve was 0.675 (95%CI 0.602~0.749,P=0.038);the sensitivity of combined diagnosis was 88.33%,the specificity was 86.25
作者
陈南耀
余丹
CHEN Nanyao;YU Dan(Department of Neurology,Haikou Fourth People's Hospital,Haikou,Hainan 571199,China;Department of Neurology,Haikou People's Hospital,Haikou,Hainan 570208,China)
出处
《中国动脉硬化杂志》
CAS
2019年第6期502-506,共5页
Chinese Journal of Arteriosclerosis
基金
海南省自然科学基金面上项目(818MS161)
