摘要
目的分析疏血通注射液的提取工艺和质量控制。方法分别采用常规提取工艺(10%生理盐水、室温条件)、优化提取工艺(浓度80%乙醇、70℃条件)及离心法提取疏血通的有效成分(地龙和水蛭),样品中次黄嘌呤含量的测定工具选用紫外分光检测器,样品的鉴别方法采用薄层色谱法。观察两种提取工艺的提取时间。结果常规提取工艺组的疏血通提取时间(1.82±0.37)h,显著长于优化提取工艺组的疏血通提取时间(1.00±0.05)h,两者间的差异有统计学意义(P<0.05)。疏血通的优化提取工艺为:浓度80%乙醇,提取温度70℃,提取时间1h,加醇量10倍;次黄嘌呤峰面积积分值RSD为1.64%,平均次黄嘌呤含量为1.25%。结论疏血通注射液的提取工艺可采用优化提取工艺,质量控制措施选用薄层色谱法(鉴别样品)、紫外分光检测法(测定样品中的次黄嘌呤含量)。
Objective Analysis Shuxuetong injection extraction process and quality control. Methods The extraction of hypoxanthine (Dioscorea zingiberensis) and Leech (Hirudo chamaejasme) by conventional extraction process (10% physiological saline at room temperature), extraction (80% ethanol, 70 ℃) Determination of the content of the tool used UV spectrophotometer detector, sample identification method using thin layer chromatography. Observe the extraction time of two extraction processes. Results The extraction time of Shuxuetong was (1.82±0.37) h, which was significantly longer than that of the optimized extraction process group (1.00±0.05) h, the difference was statistically significant (P<0.05). Shuxuetong optimized extraction process: ethanol concentration 80%, extraction temperature 70 ℃, extraction time 1h, add alcohol 10 times;hypoxanthine peak area integral value of 1.64%, the average hypoxanthine content of 1.25%. Conclusion Shuxuetong injection can be extracted using an optimized extraction process, quality control measures selected thin layer chromatography (sample identification), UV spectrophotometry (determination of the sample hypoxanthine content).
作者
顾健
王云峰
施天慧
Gu Jian;Wang Yunfeng;Shi Tianhui(Pharmacy Teaching and Research Section, Nantong Health College of Jiangsu Province, Nantong, Jiangsu, 226000, China)
出处
《当代医学》
2019年第18期91-93,共3页
Contemporary Medicine
关键词
疏血通注射液
提取工艺
质量控制
Shuxuetong injection
Extraction process
Quality control
