摘要
目的探讨全基因组及GCH1基因甲基化水平与带状疱疹神经痛的相关性。方法选择2017年6-10月在徐州医学科大学附属医院皮肤科确诊为带状疱疹且有明显神经痛症状的患者,经抗病毒和营养神经等药物治疗后疼痛完全缓解,以不影响正常睡眠为标准。最终入选36例患者及同期36例健康对照。取患者治疗前后及健康对照者外周血,斑点杂交实验检测全基因组甲基化水平的变化,甲基化DNA富集试剂盒富集GCH1基因甲基化区域,实时定量PCR技术检测GCH1基因甲基化水平的变化。采用GraphPad Prism v7.00软件对实验数据进行统计分析,带状疱疹患者治疗前后比较应采用配对t检验,而患者组与健康对照组比较采用两独立样本t检验。结果带状疱疹神经痛患者治疗前组、治疗后组及健康对照组全基因组相对甲基化水平分别为135.94 ± 2.52、144.76 ±3.48,146.84 ± 3.39,治疗前组显著低于治疗后组(t = 2.056,P < 0.05)及健康对照组(t = 2.580,P <0.05),而治疗后组与健康对照组差异无统计学意义(t = 0.429,P > 0.05).治疗前组、治疗后组及健康对照组GCH 1相对甲基化水平分别为0.65 ± 0.17,0.89 ± 0.13.0.97 ± 0.07,治疗前组显著低于治疗后组(t = 3.977,P < 0.05)及健康对照组(t = 4.648,P < 0.01),而治疗后组与健康对照组差异无统计学意义(t = 0.506,P>0.05)。结论带状疱疹神经痛患者组全基因组及GCH1基因甲基化水平降低。
Objective To analyze the correlation between herpes zoster neuralgia and the methylation status of the whole genome and GCH 1 gene. Methods From June to October in 2017, patients with confirmed herpes zoster and obvious neuralgia were selected in Department of Dermatology, The Affiliated Hospital of Xuzhou Medical University, who achieved complete remission (no effect was observed on normal sleep) of neuralgia after antiviral and neurotrophic treatment. Finally, 36 patients and 36 healthy controls were enrolled into this study. Peripheral blood samples were obtained from the healthy controls and patients before and after the treatment. Dot - blot hybridization assay was performed to determine the methylation status of the whole genome, methylated?DNA IP kit was used to enrich the methylation sites of the GCH1 gene, and real-time quantitative PCR was conducted to detect changes in methylation status of the GCH 1 gene. Statistical analysis was carried out with GraphPad Prism v7.00 software by using paired t test for the comparison of methylation status before and after the treatment, and two - sample t test for the comparison between the patient group and control group. Results The relative methylation level of the whole genome was 135.94 ± 2.52 in the patients before treatment, significantly lower than that in the patients after treatment (144.76 ± 3.48,t= 2.056, P < 0.05) and healthy control group (146.84 ± 3.39, t = 2.580, P < 0.05). However, there was no significant difference in the methylation status of the whole genome between the patients after treatment and healthy controls (t= 0.429, P > 0.05). Compared with the patients after treatment (0.89 ±0.13) and healthy control group (0.97 ± 0.07), the methylation status of the GCH1 gene significantly decreased in the patients before treatment (0.65 ± 0.17;t = 3.977, 4.648 respectively, P < 0.05,< 0.01 respectively), while no significant difference between the patients after treatment and the healthy controls(t= 0.506, P > 0.05). Conclusion The methylation s
作者
杨肖肖
郑娜娜
钟连生
潘志强
郁泽宇
Yang Xiaoxiao;Zheng Na-na;Zhong Liansheng;Pan Zhiqiang;Yu Zeyu(Department of Dermatology and Venereology,The Affiliated Hospital of Xuzhou Medical University,Xuzhou221002,Jiangsu,China;Key Laboratory of Anesthesiology,Xuzhou Medical University,Xuzhou 221002,Jiangsu,China;Morphological Research Experiment Center,Xuzhou Medical University,Xuzhou 221002,Jiangsu,China)
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2019年第6期420-424,共5页
Chinese Journal of Dermatology
