摘要
目的:研究乐尔脉胶囊对脑缺血再灌注损伤大鼠大脑皮层组织中氧化应激损伤的影响及机制。方法:将65 只大鼠随机分为假手术组(生理盐水)、模型组(生理盐水)、阳性对照组(脑心通胶囊,3.40 g/kg)和乐尔脉胶囊高、低剂量组(0.37、0.18 g/kg),每组13只。除假手术组大鼠行假手术外,其余各组大鼠均采用线栓法复制大鼠中动脉闭塞/再灌注(MCAO/R)模型。造模后24 h开始灌胃给药,每天给药1次,连续给药7 d。末次给药2 h后,采用2,3,5-氯化三苯基四氮唑(TTC)染色法测定大鼠脑缺血面积;苏木精-伊红(HE)染色后在显微镜下观察脑组织病理学变化;Western blot法检测大脑皮层组织中血红素加氧酶1(HO-1)、铜锌超氧化物歧化酶(SOD1)、锰超氧化物歧化酶(SOD2)和核因子E2 相关因子2(Nrf2)蛋白表达水平。结果:与假手术组比较,模型组大鼠脑缺血面积显著增加(P<0.05),间质重度水肿,炎性细胞浸润明显;大脑皮层组织中HO-1和SOD1蛋白表达水平显著升高(P<0.05),SOD2 和Nrf2 蛋白表达水平显著降低(P<0.05)。与模型组比较,乐尔脉胶囊高、低剂量组大鼠脑缺血面积均显著减小(P<0.05),间质水肿减轻,炎性细胞浸润减少,小胶质细胞增生减弱;大脑皮层组织中HO-1、SOD1、SOD2 和Nrf2 蛋白表达水平均显著升高(P<0.05)。结论:乐尔脉胶囊对脑缺血再灌注损伤大鼠具有一定改善作用,可能与上调皮层组织中抗氧化因子HO-1、SOD1、SOD2和Nrf2蛋白的表达有关。
OBJECTIVE:To study the effects and mechanism of Le’ermai capsule on oxidative stress injury in the cerebral cortex of cerebral ischemia-reperfusion injury rats. METHODS:Totally 65 rats were randomly divided into sham operation group (normal saline),model group (normal saline),positive control group (Naoxintong capsule,3.40 g/kg),Le’ermai capsule high-dose and low-dose groups(0.37,0.18 g/kg),with 13 rats in each group. Except that sham operation group received sham operation,and middle cerebral artery occlusion/reperfusion(MCAO/R)model was induced by suture-occluded method. 24 h after modeling,they were given relevant medicine intragastrically,once a day,for consecutive 7 d. 2 h after last administration, cerebral ischemic area was determined by TTC staining. The pathological changes of cerebral tissue were observed by HE staining. The protein expressions of HO-1,SOD1,SOD2 and Nrf2 in cerebral cortex were detected by Western blot assay. RESULTS: Compared with sham operation group,the area of cerebral ischemic was increased significantly in model group(P<0.05), interstitial edema was serious,inflammatory cell infiltration increased significantly;and the protein expression levels of HO-1 and SOD1 in cortex tissue were significantly increased (P<0.05),and the protein expression levels of SOD2 and Nrf2 were significantly decreased(P<0.05). Compared with model group,the area of cerebral ischemic area of Le’ermai capsule high-dose and low-dose groups were significantly decreased(P<0.05). The interstitial edema,inflammatory cell infiltration and microcytes proliferation were decreased. The protein expression levels of HO-1,SOD1,SOD2 and Nrf2 in the cerebral cortex tissue were significantly increased(P<0.05). CONCLUSIONS:Le’ermai capsule can improve cerebral ischemia/reperfusion injury to certain extent,and which may be associated with up-regulating the protein expression of antioxidant factors HO-1,SOD1,SOD2 and Nrf2 in the cortex.
作者
池宁娟
刘明义
冯娟
石小鹏
杨志福
文爱东
CHI Ningjuan;LIU Mingyi;FENG Juan;SHI Xiaopeng;YANG Zhifu;WEN Aidong(Dept. of Pharmacy,the First Affiliated Hospital of Air Force Military Medical University,Xi’an 710032,China)
出处
《中国药房》
CAS
北大核心
2019年第11期1525-1529,共5页
China Pharmacy
基金
国家自然科学基金面上资助项目(No.81573549)
