摘要
通过优化微波消解用酸种类、最高温度及保持时间等条件,建立微波消解-火焰光度计测定植物中全钾的新方法。结果表明,采用6mLHNO3+2mLH2O2酸系,微波消解最高温度160℃,保持时间15min的优化消解方法,测定的检出限0.002mg/g;测定两种植物标准物质的全钾含量均在其证书标准值范围内;测定水稻植株样品的全钾含量加标回收率97.5%~103.0%,相对标准偏差0.63%~2.27%。该方法适用于植物不同部位全钾的测定,操作简便安全,结果准确可靠。
A new method for the determination of total potassium in plants by microwave digestion-flame photometry was established by optimizing the conditions of digested acid, maximum digestion temperature and retention time of microwave digestion. The results showed that the optimal digestion condition was as follows:6 mL HNO3+2 mL H2O2 acid system, maximum digestion temperature 160 ℃ and retention time 15 min, and the detection limit was 0.002 mg/g. The results of the total potassium content of two plant reference materials determined by the optimal digestion procedure showed that the data was within the range of the certificate standard values. The recovery rates of total potassium contents of rice plant samples tested by the optimal digestion procedure were 97.5%~103.0% and the relative standard deviation was 0.63%~2.27%. The results suggested that this method is suitable for the determination of total potassium in different parts of plants, and the operation is simple and safe, and the result is accurate and reliable.
作者
陶曙华
龚浩如
陈祖武
陈英姿
苗雪雪
王洁敏
TAO Shu-hua;GONG Hao-ru;CHEN Zu-wu;CHEN Ying-zi;MIAO Xue-xue;WANG Jie-min(Hunan Rice Reserch Institute,Changsha 410125,China;Key Laboratory of Genetics and Breeding of Middle and Lower Yangtze River,MOA China,Changsha 410125,China)
出处
《湖北农业科学》
2019年第10期142-145,共4页
Hubei Agricultural Sciences
基金
国家重点研发计划项目(2018YFD0301003)
关键词
微波消解
火焰光度法
植物
全钾
microwave digestion
flame photometry
plant
total potassium
