摘要
目的:建立测定博落回茎叶中非生物碱N-对香豆酰酪胺含量的方法,为博落回茎叶微粉兽药质量控制提供新的评价指标。方法:采用HPLC,色谱柱为资生堂MG3C18(250mm×4.6mm,5μm);流动相为甲醇∶水(40∶60)梯度洗脱[1];检测波长为290nm;流速为1.0mL·min^-1;柱温为35℃。结果:N-对香豆酰酪胺的含量在0.0775~0.4704mg(r=0.9996)与峰面积呈良好的线性关系,该测定N-对香豆酰酪胺含量方法的平均回收率为99.45%。结论:所建立的方法操作简单,方便快捷,重复性好,适合用于测定博落回茎叶中N-对香豆酰酪胺的含量。
Objective:To establish a method for the determination of non-alkaloid N-p-Coumaroyltyramine in stems and leaves of Macleaya cordata.This study provided a new evaluation index for the quality control of micropowder veterinary drugs in the stems and leaves of M.cordata.Methods:HPLC method was used in this paper,the chromatographic column was Shiseido MG3 C18(250 mm×4.6 mm,5μm),the mobile phase was methanol∶water(40∶60)gradient elution[1],the detection wavelength was 290 nm,the flow rate was 1.0 mL·min^-1,and the column temperature was 35℃.Results:The content of N-p-coumaroyltyramine ranged from 0.077 5 mg to 0.470 4 mg(r=0.999 4),which showed a good linear relationship with the peak area.The average recovery of the method for the determination of N-p-coumaroyltyramine was 99.45%.Conclusion:The method is simplicity of operator,convenient and reproducible.It is suitable for the determination of N-p-coumaroyltyramine in the stems and leaves of M.cordata.
作者
曾诚
曾建国
Zeng Cheng;Zeng Jian-guo(Hunan Key Laboratory of Chinese Veterinary Medicine,Changsha,Hunan 410128,China;Hunan Agricultural University,Changsha,Hunan 410007,China)
出处
《中国现代中药》
CAS
2019年第5期622-624,628,共4页
Modern Chinese Medicine
基金
中药材全产业链发展(2016SK3002)
