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不同菌种菊苣根发酵提取物的挥发性成分分析及应用 被引量:2

Analysis and application of volatile components in extracts from chicory roots fermented by different strains
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摘要 采用同时蒸馏萃取/气相色谱-质谱联用(SDE/GC-MS)法对菊苣根长孢诺德酵母菌(Lodderomyces elongisporus)发酵提取物(A)和菊苣根短小芽孢杆菌(Bacillus pumilus)发酵提取物(B)的挥发性成分进行分析,并使用峰面积归一化法计算各成分相对百分含量。结果表明,A和B中共分析鉴定出44种挥发性成分,其中A中40种,B中31种,二者相同成分27种。成分分析结果表明,A和B中主要挥发性成分为棕榈酸、糠醛、3-甲基戊酸、2-乙酰基呋喃等。B中的挥发性成分种类较少,但主要致香成分含量高,且对烟草吸味起副作用的成分含量更小。卷烟加香试验结果表明,菊苣根发酵提取物添加到卷烟中,具有掩盖杂气,纯净余味的作用,并使卷烟香气丰富饱满,烤甜香明显,烟气柔和透发,总体表现B优于A,添加比例以0.05%最佳。 The volatile flavor components in extracts from chicory roots fermented by Lodderomyces elongisporus (A) and Bacillus pumilus (B) were determined by SDE/GC-MS, and the relative content of each components was detected by the normalization method of peak area. The results showed that 44 compounds were identified, including 40 compounds in A and 31 compounds in B, and there were 27 identical components in A and B. The results of component analysis showed that the main volatile components in A and B were palmitic acid, furfural, 3-methyl valeric acid and 2-acetyl furan etc. Although the species of volatile components in B were less, the content of main aroma-producing components was higher and the content of components which had side effects on tobacco smoking was less. Cigarette flavoring tests showed that the fermentation extract of chicory root added to cigarettes had the effect of covering up the offensive odor, purifying the aftertaste, enriching and satiating the aroma of cigarettes, with obvious roasting sweetness and soft, transparent smoke. The overall performance of B was better than A, and the optimal addition proportion was 0.05%.
作者 常健 徐世涛 黄静 刘劲云 王夸平 徐重军 陈媛 王乃定 段焰青 李源栋 杨培香 CHANG Jian;XU Shitao;HUANG Jing;LIU Jinyun;WANG Kuaping;XU Chongjun;CHEN Yuan;WANG Naiding;DUAN Yanqing;LI Yuandong;YANG Peixiang(Yunnan Tobacco Industrial Hi-Tech Material Co.,Ltd.,Kunming 650106,China;Technology Center,China Tobacco Yunnan Industrial Co.,Ltd.,Kunming 650202,China)
出处 《中国酿造》 CAS 北大核心 2019年第5期199-203,共5页 China Brewing
基金 云南中烟工业有限责任公司重点项目(2017CP04)
关键词 菊苣根 长孢诺德酵母菌 短小芽孢杆菌 发酵 挥发性成分 卷烟加香 chicory roots Lodderomyces elongisporus Bacillus pumilus fermentation volatile components cigarette flavoring
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