摘要
目的:建立氧化应激骨骼肌细胞模型,观察黄芪多糖(APS)对其的影响,为今后的实验提供依据。方法:过氧化氢(H2O2)分100μM、200μM、300μM、400μM 4组浓度干预骨骼肌细胞,每组6个复孔,分别培养12 h和24 h,MTT法检测增殖率;用100μM H2O2制作氧化应激骨骼肌细胞模型(模型组),给予0.2 mg/mL APS干预(干预组),并设正常组,每组6个复孔,培养24 h,MTT法检测增殖率,流式细胞仪检测凋亡率、活性氧簇(ROS)水平。结果:与正常组比较,100μM以上浓度的H2O2均有统计学意义(P<0.01),随着H2O2浓度的增加,骨骼肌细胞的增殖率显著下降(P<0.01)。与正常组比较,模型组的ROS水平及凋亡率均升高(P<0.01);干预组的ROS水平及凋亡率均下降(P<0.01),与模型组比较差异有统计学意义(P<0.01)。结论:100μM H2O2是氧化应激骨骼肌细胞模型的有效浓度。APS具有良好的抗氧化性,具有改善肌肉减少症的作用。
Objective: To establish the model of oxidative stress of skeletal muscle cells, and to observe the effect of astragalus polysaccharides(APS) on it, thus to provide a basis for future experiments. Methods: The skeletal muscle cells were treated with hydrogen peroxide(H 2O 2) in groups of 100 μM, 200 μM, 300 μM and 400 μM. Each group was given 6 replicate wells and cultured for 12 h and 24 h respectively. MTT was used to detect the proliferation rate. The models of oxidative stress of skeletal muscle cells(the model group) was made with 100 μM H 2O 2, and was treated with 0.2 mg/mL APS(the intervention group). The normal group was set up with 6 duplicate wells in each group, cultured for 24 h. MTT was used to detect the proliferation rate. Rate flow cytometry was applied to detect the apoptotic rate and reactive oxygen species(ROS) levels. Results: The concentration of H 2O 2 above 100 μM was statistically significant compared to that in the normal group( P < 0.01). As the concentration of H 2O 2 increased, the apoptotic rate in skeletal muscle cells decreased significantly( P <0.01). Compared to the normal group, the ROS level and apoptotic rate of the model group were significantly increased( P <0.01);the ROS level and apoptotic rate of the intervention group were significantly decreased( P <0.01);there was a significant difference in it when compared to the model group( P <0.01). Conclusion: 100 μM H 2O 2 is an effective concentration of oxidative stress skeletal muscle cell model. APS has better action of anti-oxidation and it can improve muscle reduction.
作者
邓聪
杨志敏
徐福平
孙玉姣
陈玉状
赖艳娴
岑美婷
DENG Cong;YANG Zhimin;XU Fuping;SUN Yujiao;CHEN Yuzhuang;LAI Yanxian;CEN Meiting(Nansha Hospital of Guangzhou First People's Hospital, Guangzhou 511457, China;The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou 510120, China)
出处
《中医药信息》
2019年第3期80-82,共3页
Information on Traditional Chinese Medicine
基金
广州市第三批优秀中医临床人才研修项目(No.GZYC03201602)
关键词
黄芪多糖
骨骼肌
氧化应激
活性氧簇
Astragalus polysaccharides
Skeletal muscle
Oxidative stress
Reactive oxygen species
