摘要
为明确缅甸流行区间日疟原虫红内期疫苗候选抗原Duffy结合蛋白(DBP)的基因多态性特点,提取20例缅甸流行区(Bilin和Paletwa)间日疟原虫基因组DNA,通过PCR扩增和测序检测,参考Mandalay流行区(缅甸)相应序列,对pvdbp-Ⅱ基因进行多态性分析。结果表明,各流行区pvdbp-Ⅱ基因的π值分别为0.002、0.009和0.002,且d_N/d_S均大于1。中性检验表明各流行区pvdbp-Ⅱ基因处于阳性选择;pvdbp-Ⅱ基因在Bilin和Mandalay流行区存在基因重组,各流行区群体间存在不同程度的遗传分化,H14是21种不同的单倍型中出现频率最高的单倍型。Pvdbp-Ⅱ基因存在多态并经历阳性选择,提示其作为红内期疫苗候选靶点;H14高频率的出现,提示以其为基础研制pvdbp-Ⅱ基因作为红内期疫苗候选抗原的可能性。
To clarify the genetic diversity feature of a blood stage vaccine candidate antigen, Duffy binding protein(DBP), the genomic DNA was extracted from 20 P. vivax infected patients′ blood samples from malaria epidemic areas(Bilin and Paletwa) in Myanmar. Pvdbp-Ⅱ gene was amplified by PCR and sequenced. Combined with the sequence of Mandalay, which was achieved from GenBank database, the polymorphism analysis of pvdbp-Ⅱ gene was carried out. The results showed that π value of pvdbp-Ⅱ gene was 0.002, 0.009 and 0.002, respectively and d_N/d_S>1 was detected in all studied epidemic areas in Myanmar. Positive diversifying selection was detected by neutral tests of pvdbp-Ⅱ gene in all studied epidemic areas. Recombination occurred in Bilin and Mandalay endemic areas. Different degrees of genetic differentiation existed among populations in various endemic areas. H14 as the most frequent haplotype was found in 21 different haplotypes. Pvdbp-Ⅱ gene showed genetic diversity and had experienced positive diversifying selection, indicating that it was a candidate target for blood stage vaccine;H14 was the most frequent haplotype, which suggested the possibility of developing pvdbp-Ⅱ gene as a candidate antigen of endoerythrocytic stage vaccine based on this haplotype.
作者
韦焕苹
闫妍
洪明阳
朱晓彤
曹雅明
WEI Huan-Ping;YAN Yan;HONG Ming-Yang;ZHU Xiao-Tong;CAO Ya-Ming(Department of Immunology,College of Basic Medical Science,China Medical University,Shenyang,Liaoning 110122)
出处
《寄生虫与医学昆虫学报》
CAS
2019年第1期7-12,共6页
Acta Parasitologica et Medica Entomologica Sinica
基金
辽宁省教育厅课题(No.LQNK201727)资助
