摘要
目的:探讨尼莫地平治疗大鼠颈动脉狭窄所致术后认知功能障碍(POCD)的效果。方法:选取54只SD大鼠采用随机数字表法分为假手术组、狭窄组及尼莫地平组(n=18)。尼莫地平组大鼠于造模成功2周后腹腔注射1mg·kg^-1尼莫地平,假手术组及狭窄组给予等量生理盐水,连续4周,每天1次。分别于治疗前、治疗后2周、治疗后4周检测大鼠空间学习记忆能力,海马神经递质含量,海马组织结构变化以及Bax,Bcl-2,裂解的含半胱氨酸的天冬氨酸蛋白水解酶3、9蛋白表达。结果:与狭窄组比较,尼莫地平组治疗后相应时间点狭窄率降低、逃避潜伏期缩短(P<0.01),平均平台象限游泳距离百分比增加(P<0.01);治疗4周后与狭窄组比较,尼莫地平组大鼠海马神经元形态较为一致,NE、DA及5-HT含量升高(P<0.01),Bax及含半胱氨酸的天冬氨酸蛋白水解酶3、9表达量下调(P<0.01),Bcl-2表达量上调(P<0.01)。结论:尼莫地平可通过抑制神经元凋亡改善颈动脉狭窄导致的大鼠POCD。
Objective: To explore effect of nimodipine on post-operative cognitive dysfunction (POCD) in rats with carotid artery stenosis. Methods: Fifty-four rats were randomly divided into sham operation group, stenosis group and nimodipine group with 18 rats in each group. After two weeks, the rats were injected with 1 mg·kg -1 nimodipine intraperitoneally in nimodipine group, and the rats in the sham operation group and the stenosis group were given equal amount of saline for 4 weeks. The spatial learning and memory ability, hippocampal neurotransmitter content, hippocampal tissue structure, expression of B lymphocyte tumor-2 (Bcl-2), Bcl-2 related X protein (Bax), cleaved aspartic acid protein hydrolase 3 (Caspase 3) and 9 protein were detected before treatment, 2 weeks and 4 weeks after treatment. Results: Compared with the stenosis group, the stenosis rate and escape latency were decreased( P <0.01), average platform quadrant swimming distance percentage was increased( P <0.01) in the nimodipine group before and after treatment at the same time. After 4 weeks treatment,compared with the stenosis group, morphology of hippocampal neurons was a more consistent. The contents of NE, DA and 5-HT were increased ( P <0.01), and the expressions of Bax, cleaved caspase 3 and 9 were decreased ( P <0.01), and the expression of Bcl-2 was increased in the nimodipine group ( P <0.01). Conclusion: Nimodipine can improve POCD in rats with carotid artery stenosis via inhibition neurons apoptosis.
作者
郑文婧
郄晓娟
郑文芳
王忠义
张同军
ZHENG Wenjing;QIE Xiaojuan;ZHENG Wenfang;WANG Zhongyi;ZHANG Tongjun(Department of Anesthesiology,Hengshui People s Hospital,Hengshui 053000,China;Department of Anesthesiology,the Third Hospital of Hebei Medical University,Shijiazhuang 050000,China;Department of Anesthesiology,Maternal and Child Health Hospital of Hengshui City,Hengshui 053000,China)
出处
《东南大学学报（医学版）》
CAS
2019年第2期291-296,共6页
Journal of Southeast University(Medical Science Edition)
基金
河北省2014年度医学科学研究重点课题计划(ZL20140225)
关键词
尼莫地平
颈动脉狭窄
术后认知功能障碍
大鼠
nimodipine
carotid artery stenosis
post-operative cognitive dysfunction
rats
