摘要
目的采用复杂网络方法探究温心方治疗冠状动脉狭窄的作用机制,为中药新药研发提供参考。方法利用多种中医药知识、药物靶点、疾病靶点数据库筛选温心方有效成分,构建温心方成分-靶点网络及冠状动脉狭窄疾病-靶点网络,采用Cytoscape软件整合并构建成分-疾病-靶点蛋白质相互作用(PPI)网络,对该网络进行拓扑分析及核心靶点筛选,然后进行生物过程及信号通路的富集分析。结果温心方含148种药物成分,可作用于226个靶点基因,冠状动脉狭窄共计471个疾病靶点,其中66个与温心方作用靶点重合。PPI网络及拓扑分析显示,温心方作用于冠状动脉狭窄的核心靶点380个,涉及ErbB信号通路、B细胞抗原受体信号通路、HIF-1信号通路、TGF-β信号通路、凝血活动、G1/S细胞周期转换、Wnt信号通路等多种生物过程及信号通路。结论温心方治疗冠状动脉狭窄多靶点、多途径的作用网络,提示其可能通过抗凋亡、调控细胞周期、抗炎、抗凝、促血管新生、保护内皮细胞发挥治疗作用。
Objective To explore the mechanism of Wenxin Decoction in treating coronary artery stenosis based on complex network method;To provide references for drug development. Methods Active ingredients in Wenxin Decoction were screened by using a variety of TCM knowledge, drug targets, and disease target databases, and Wenxin Decoction component-target network and coronary stenosis-target network were constructed. Cytoscape software was used to integrate and construct a component-disease-target protein-protein interaction (PPI) network, and the network was under topological analysis and core target screening. Enrichment analysis of biological processes and signaling pathways were conducted. Results A total of 148 ingredients in Wenxin Decoction were found to act on 226 target genes. Coronary artery stenosis had a total of 471 disease targets, of which 66 coincided with Wenxin Decoction targets. PPI network and topological analysis showed that Wenxin Decoction acted on 380 core targets of coronary stenosis, involving various biological processes and signaling pathways such as ErbB signaling pathway, B cell antigen receptor signaling pathway, HIF-1 signaling pathway, TGF-β signaling pathway, clotting activity, G1/S cell cycle transition and Wnt signaling pathway. Conclusion The multi-target and multi-path network of Wenxin Decoction for treating coronary artery stenosis implies that it may play a therapeutic role through anti- apoptosis, regulation of cell cycle, anti-inflammatory, anticoagulation, angiogenesis, and protection of endothelial cells.
作者
李金霞
曹洪欣
LI Jinxia;CAO Hongxin(Institute of Basic Theory for Chinese Medicine,China Academy of Chinese Medical Sciences,Beijing 100700,China;China Association of Chinese Medicine,Beijing 100029,China)
出处
《中国中医药信息杂志》
CAS
CSCD
2019年第6期101-105,共5页
Chinese Journal of Information on Traditional Chinese Medicine
基金
唐仲英中药基金会资助项目(2013-2023)
关键词
温心方
冠状动脉狭窄
网络药理学
靶点
信号通路
Wenxin Decoction
coronary artery stenosis
network pharmacology
targets
signaling pathway
