摘要
以紫甘蓝为原料,首先对紫甘蓝花青素苷进行定性分析,鉴定16种矢车菊色素苷;然后对矢车菊色素苷的提取条件进行优化。结果表明,最佳提取条件为含1%盐酸的50%乙醇溶液、料液比1∶10(g/mL);利用AB-8型大孔树脂对矢车菊色素苷进行初步纯化后,通过高温酸水解的方法获得游离态矢车菊色素粗品;再利用C18柱层析的方法纯化得到矢车菊色素单体;最后通过结晶沉淀方法获得纯度大于99%的矢车菊色素单体粉末。
In this experiment we identified 16 cyanidin glycosides from purple cabbage. Cyanidins were extracted from purple cabbage under the optimized conditions as follows: 50% ethanol containing 1% hydrochloric acid as extraction solvent, and a solid-to-solvent ratio of 1:10(g/mL). The extract was purified with macroporous resin AB-8 and then hydrolyzed with acid at high temperature to free cyanidin glycosides. Furthermore, the hydrolysate was purified by C18 column chromatography, yielding cyanidin monomers with 99% purity.
作者
蒋晓岚
石渝凤
付周平
成琳
刘亚军
高丽萍
夏涛
JIANG Xiaolan;SHI Yufeng;FU Zhouping;CHENG Lin;LIU Yajun;GAO Liping;XIA Tao(School of Tea and Food Technology, Anhui Agricultural University, Hefei 230036, China;School of Life Science, Anhui Agricultural University, Hefei 230036, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第10期257-264,共8页
Food Science
基金
安徽农业大学稳定与引进人才项目(yj2017-37)
安徽省自然科学基金项目(1708085QC51)
关键词
紫甘蓝
矢车菊色素
分离纯化
结晶
purple cabbage
cyanidin
extraction and purification
crystallization
