摘要
针对移液器人工校准中存在的按压不稳、温度偏差、数据无法批量处理等缺陷,提出了一种基于模拟人工按压的移液器容量自动校准方法。通过测量移液器按钮2个停止点的压力值,获取近似按压的距离;分析数据并与人工操作进行比对,得出模拟手动按压的最佳按压距离。开发了一套同时校准4支移液器容量的自动化装置,采用机械手和Lab VIEW软件智能控制取液、排液、称量、数据处理等工作,实现了自动校准。研究结果表明,与人工操作相比,重复性减小了0. 0%～0. 9%,相对误差减小了0. 1%～0. 7%。
Aim at the shortcomings of manual calibration such as instability of the press, temperature deviation and data that can not been processed in batches, an automatic calibration method for pipette capacity based on simulated manual press was proposed. By measuring the pressure at the two stop points of the button of the pipette, the approximate pressing distance can be obtained;comparing and analyzing data with manual operation, the optimum press distance of simulated manual press was obtained. An automatic device for simultaneous calibration of 4 pipettes was developed. Those works were intelligently controlled including extraction, discharge, weighing, and data processing adopting manipulator and LabVIEW software, fully-automatic calibrating were realized. Research results show that comparing to manual operation, the repeatability reduces 0.0%~0.9%, and the relative error decreases by 0.1%~0.7%.
作者
赵玉晓
谢玄达
孙斌
张竟月
文英杰
ZHAO Yu-xiao;XIE Xuan-da;SUN Bin;ZHANG Jing-yue;WEN Ying-jie(College of Modern Science and Technology, China Jiliang University, Hangzhou, Zhejiang 310018, China;National Institute of Metrology, Beijing 100029, China;Hangzhou Institute of Calibration andTesting for Quality and Technical Supervision, Hangzhou, Zhejiang 310019, China)
出处
《计量学报》
CSCD
北大核心
2019年第3期392-396,共5页
Acta Metrologica Sinica
基金
国家自然科学基金(51475440)
国家质量监督检验检疫总局科技计划项目(2017QK123)
关键词
计量学
移液器
自动校准
小容量计量
不确定度
metrology
pipette
automatic calibrating
small capacity metrology
uncertainty
