摘要
为发掘新的抗南方锈病基因资源,本研究以感病自交系黄早四为母本、抗病自交系W456为父本,构建F2群体并开展抗病基因定位研究。采用人工接种鉴定的方法对两个亲本、F1、F2群体及对照材料进行表型鉴定和遗传分析。利用均匀覆盖10条染色体的200个SSR标记,分析240个F2单株的基因型并构建含有200个SSR位点的遗传连锁图,连锁图总长度3331 cM,标记间平均距离16.6 cM。使用QTL IciMapping V4.1软件中的完备区间作图法对抗病QTL进行分析,共检测到6个控制南方锈病的QTL:qSCR3、qSCR7、qSCR8-1、qSCR8-2、qSCR9和qSCR10,邻近标记分别为umc2105和umc1729、umc1066和bnlg2271、umc1904和umc1984、umc1984和bnlg1651、umc1957和bnlg1401、umc2034和umc1291,分别位于3、7、8、9和10号染色体上,其中8号染色体上有两个位点,标记区间长度在5～19 cM之间。单个QTL的表型贡献率在2.61%～24.19%之间,可以解释表型总变异的62.3%,其中3个QTL贡献率大于10%,位于10号染色体上的qSCR10贡献率最大,可解释表型变异的24.19%。通过对目标区间标记加密,将该位点的定位区间进一步缩小到2.51 cM内,与两侧标记的距离分别是2.15 cM和0.36 cM。初步定位得到10号染色体上存在抗南方锈病的主效QTL,可为抗病品种的培育提供参考。
Southern corn rust has become an economically important disease that affects maize yield in China.To explore new genetic resources for resistance to southern rust,we developed F1 hybrids and F2 population by a cross between a highly resistant inbred line W456 and susceptible Huangzaosi. These genetic materials were subjected for genetic analysis and QTL mapping of resistance genes. A framework of linkage map comprising 200 SSR markers was constructed which expands 3331 cM of the maize genome with an average marker interval of 16.6 cM. QTL mapping for resistance was conducted using software IciMapping V4.1 package for inclusive composite interval mapping. As a result,six QTLs including qSCR3,qSCR7,qSCR8-1,qSCR8-2,qSCR9 and qSCR10,were detected on chromosomes 3,7,8,9 and 10,which were flanked by markers umc2105 and umc1729,umc1066 and bnlg2271,umc1904 and umc1984,umc1984 and bnlg1651,umc1957 and bnlg1401,and umc2034 and umc1291,respectively. The phenotypic variance explained(PVE)ranged from 2.61% to 24.19%,explaining62.3% of the total phenotypic variation. Notably,qSCR10 on chromosome 10 accounted for 24.19% of the phenotypic variation,thus being a major QTL responsible for resistance to southern rust in maize. Moreover,this resistance locus was further delimited by new flanking markers to a 2.51 cM genetic interval. Thus,this work accomplished the preliminary mapping of resistance loci,which might be valuable in breeding for resistant varieties against Southern corn rust.
作者
陈文娟
路璐
李万昌
张小杰
孙素丽
朱振东
王晓鸣
段灿星
CHEN Wen-juan;LU Lu;LI Wan-chang;ZHANG Xiao-jie;SUN Su-li;ZHU Zhen-dong;WANG Xiao-ming;DUAN Can-xing(Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and GeneticImprovement, Beijing 100081;College of Life Science, Henan Normal University, Xinxiang 453007)
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2019年第3期521-529,共9页
Journal of Plant Genetic Resources
基金
作物种质资源保护子项(2018NWB036-12)
中国农业科学院科技创新工程
科技部
财政部国家科技基础条件平台(NICGR2016-008)~~
